DescriptionViPrimePLUS Lyophilized qPCR Master Mix is next generation first choice for a fast and easy real-time PCR reaction set up. The master mix is in freeze-dried form which produces stable preparation at room temperature. The freeze-dried master mix contains pure Taq DNA polymerases, MgCl2 and buffers components at optimal concentrations. ViPrimePLUS Lyophilized qPCR Master Mix has same optimal performance as ViPrimePLUS qPCR Master Mix and ViPrimePLUS qPCR Green Master Mix. The lyophilized qPCR master mix has highest sensitivity and specificity to detect the extremely low abundance transcripts with high efficiency. ViPrimePLUS Lyophilized qPCR Master Mix is used with supplied re-suspension buffer and ROX dye; hence hardware platforms that need ROX as a passive reference dye can be used in standard cycling conditions.

Features

• Convenient and rapid real time PCR reaction set up
• Lyophilized form – room temperature stable preparation
• Reliable quantification of low copy number targets
• High PCR sensitivity and specificity
• Compatible with most of the real-time PCR platforms

Kit Component

• 3 x Lyophilized qPCR MasterMix
• 1 x Lyophilized ROX
• 4 x Re-suspension buffer

Master Mixes Selection Guidelines for System CompatibilityTo calibrate a real-time PCR reaction, the various formulations of Master Mixes are available for most of the platforms.

Real-time PCR platforms	ROX re-suspension volume	ROX addition per tube of master mix
Applied Biosystems 7000, 7700, 7900, 7300 StepOne, StepOnePLUS and ViA7 platforms, Roche capillary Lightcyclers.	100µl	20µl
All Stratagene platforms	200µl	15µl
Applied Biosystems 7500 platform	700µl	10µl
All other machines	Not Required	Not Required

Ordering Information

Catalog No	Description	Pack Size
QLMM05	ViPrimePLUS Lyophilized qPCR Master Mix	150 reactions

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ViPrimePLUS Lyophilized qPCR Master Mix

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。