DescriptionAvian Myeloblastosis Virus (AMV) Reverse Transcriptase is an RNA-dependent DNA polymerase (aß holoenzyme) with molecular weight of 157 kDa. It synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.
Concentration20u/μl
Assay Condition 50mM Tris- HCl (pH8.3), 6mM MgCl2, 40mM KCl, 4mM DTT, 0.5mM [ 3H]-TTP (10-20c/m/pmol), 0.4mM Poly(rA)•(dT)12-18, in a reaction volume of 25μl.
Supplied With10X Buffer AMV-RT 250mM Tris-HCl (pH 8.3), 500mM KCl, 50mM MgCl2 and 20mM DTT. Store at -70˚C.
Storage Buffer200mM Potassium Phosphate (pH 7.2), 0.2% TritonTM X-100, 2mM DTT and 50% glycerol. Store at -70˚C for long term.Store at -20˚C for short term (1 month)..
Thermal Inactivation 80˚C for 10 minutes.
Unit Definition 1u is defined as the amount of enzyme that is required to incorporate 1nmol of dMTP into an acid-insoluble material in 10 minutes at 37˚C using Poly(rA)•(dT)12-18 as a template primer.
Features
- First strand synthesis of cDNA.
- Synthesis of cDNA for cloning.
- cDNA labelling.
- Primer extensions and RNA sequencing
- RT-PCR
- Dideoxy sequencing of DNA and RNA.
Quality Control All preparations are assayed for contaminating endonuclease, exonuclease and non-specific RNase activities.
Ordering Information
| Catalog No | Description | Pack Size |
|---|---|---|
| ME2301 | AMV Reverse Transcriptase (Recombinant) | 500u |
| ME2302 | AMV Reverse Transcriptase (Recombinant) | 2500u |
DownloadManual
AMV Reverse Transcriptase (Recombinant)
PublicationThis Product Has Been Used In:Lim et al. (2012) Co-administration of Avian Influenza Virus H5 Plasmid DNA with Chicken IL-15 and IL-18 Enhanced Chickens Immune Responses. BMC Vet Res8: 132.
VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。
