Concentration20u/μl 5 " . . . G↓A AT T C . . . 3 "3 " . . . C T TA A↑G . . . 5 "

Storage Buffer10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition20u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation65˚C for 20 minutes.

Quality Control AssaysLigation/ Recutting AssayAfter 20-fold overdigestion with EcoR I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of EcoR I for 16 hours at 37˚C.

Ordering Information

Catalog No	Description	Pack Size
FDRE1260	EcoR I	100 preps

 

DownloadsManualEcoR I

PublicationThis Product Has Been Used In:Siti-Hasmah, M., Hwe-San, L., Xiao-Ying, C., Massawe, F., Abdul-Rahman, O. (2017). Single Nucleotide Modification of VP2 Gene of Highly Virulent Infectious Bursal Disease Virus for Effective Expression Strategy in Tobacco. . Open Access Journal of Microbiology & Biotechnology, Vol. 2, No. 1 (2017).

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。