Concentration20u/μl 5 " . . . C↓C AT G G . . . 3 "3 " . . . G G TA C↑C . . . 5 "

Storage Buffer10mM Tris-HCl (pH 7.5), 50mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition20u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation65˚C for 20 minutes.

Quality Control AssaysLigation/ Recutting AssayAfter 20-fold overdigestion with Bsp19 I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of Bsp19 I for 16 hours at 37˚C.

Ordering Information

Catalog No	Description	Pack Size
FDRE1190	Bsp19 I (Nco I)	25 preps

 

DownloadsManualBsp19 I (Nco I)

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。