Concentration10u/μl 5 " . . . A↓G AT C T. . . 3 "3 " . . . T C TA G↑A . . . 5 "

Storage Buffer10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 1mM DTT, 200μg/ml BSA and 50% glycerol.

Unit Definition10u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal InactivationNone.

Quality Control AssaysLigation/ Recutting AssayAfter 10-fold overdigestion with Bgl II, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Bgl II for 16 hours at 37˚C.

Ordering Information

Catalog No	Description	Pack Size
FDRV1144	Bgl II	50 preps

 

DownloadsManualBgl II

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。