Concentration5u/μl 5 " . . . A↓C C G G T . . . 3 "3 " . . . T G G C C↑A . . . 5 "

Storage Buffer10mM Tris-HCl (pH 7.5), 250mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol.

Unit Definition5u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation65˚C for 20 minutes.

Quality Control AssaysLigation/ Recutting AssayAfter 5-fold overdigestion with AsiG I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AsiG I for 16 hours at 37°C.

Ordering Information

Catalog No	Description	Pack Size
FDRE1126	AsiG I (Age I)	25 preps

 

DownloadsManualAsiG I (Age I)

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。