Concentration5-10u/μl5"…G↓GYRCC…3" 3"…CCATG↑G…5"

Reaction Conditions 1X Buffer AccBII 10mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation65°C for 20 minutes.

Ligation / Recutting Assay After 10-fold overdigestion with AccB1I, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AccB1I for 16 hours at 37°C.Supplied with 10X Buffer AccB1I, 10X Buffer UB and Viva Buffer A (Diluent) *High enzyme concentration may result in Star Activity

Ordering Information

Catalog No	Description	Pack Size
RE1106	AccB1 I {HgiCI}	500u

 

DownloadsManualAccB1 I {HgiCI}

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。