DescriptionThe GF-1 Bacterial DNA Extraction Kit provides a rapid and efficient method for purification of high molecular weight genomic DNA from either Gram-negative or Gram-positive bacteria. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance the binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.

Features

• Suitable for both Gram-negative or Gram-positive bacteria
• Yields up to 20μg of DNA
• No organic-based extraction required
• Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.

Kit Components

• Buffer R1
• Buffer R2
• Buffer BG
• Wash Buffer (concentrate)
• Elution Buffer
• Proteinase K

Ordering Information

Catalog No	Description	Pack Size
GF-BA-050	GF-1 Bacterial DNA Extraction Kit	50 preps
GF-BA-100	GF-1 Bacterial DNA Extraction Kit	100 preps

DownloadManual

GF-1 Bacterial DNA Extraction Kit

Stability Test Report

GF-1 Bacterial DNA Extraction Kit

PublicationThis Product Has Been Used In: Ekprasert J et al. (2020) Investigating mechanical properties and biocement application of CaCO3, precipitated by a newly-isolated Lysinibacillus sp. WH using artificial neural networks, Scientific Reports,10:16137.Erkihun A & Kausalya R (2020) MCR-1 Gene Encoded Colistin-Resistant Escherichia coli in Raw Chicken Meat and Bean Sprouts in Malaysia, International Journal of Microbiology, Article ID 8853582.Eshamah HL et al. (2020)Extent of pathogenic and spoilage microorganisms in whole muscle meat, meat products and seafood sold in Libyan market, Open Veterinary Journal,10(3):276-288.Fatin Aina Zulkhairi Amin et al. (2020). Probiotic Properties of Bacillus Strains Isolated from Stingless Bee (Heterotrigona itama) Honey Collected across Malaysia,. Int. J. Environ. Res. Public Health, 17, 278.Nonzee H et al. (2020) Interaction Study of Pasteurella Multocida with Culturable Aerobic Bacteria Isolated from Porcine Respiratory Tracts using Coculture in Conditioned Media, Research Square,https://doi.org/10.21203/rs.3.rs-36993/v1Pongrawee N & Neelawan P (2020)Distribution and expression of virulence genes in potentially pathogenic bacteria isolated from seafood in Thailand, CyTA – Journal of Food 18:1, 753-763.Virun Vichaibun & Panan Kanchanaphum (2020) Quantitative LAMP and PCR Detection of Salmonella in Chicken Samples Collected from Local Markets around Pathum Thani Province, Thailand, International Journal of Food Science, Article ID 8833173.in vitro antimicrobial assessment on lactic acid bacteria isolated from common freshwater fishes,. Asia-Pacific Journal of Molecular Biology and Biotechnology, 27(2):18-25.Mankai H et al (2019) Complete Genome Sequence and Methylome Analysis of Thermoactinomyces vulgaris 2H, . Microbiology Resource Announcements, 8:e00657-19.Tung HJ et al. (2019)cDNA-SSR Markers for Molecular Epidemiology of Ganoderma boninense,. , Journal of Oil Palm Research, 31(2):220-237. Boonyayatra, S., Tharavichitkut, P., Oliver, S.P. (2018). Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand, Turkish Journal of Veterinary and Animal Sciences, Vol. 42, 73-81 (2018).Community structures of total bacterial DNA, cultivable bacteria and prototrophs in bulk soil and rhizospheres . Can, H.Y., Elmali, M., Ergün, Y. (2017).Methicillin-resistant Staphylococcus aureus in milk from dairy cows with chronic mastitis, Eurasian Journal of Veterinary Sciences, Vol. 33, No. 4, 255-259 (2017).Daniel, D.S., Gan, H.M., Lee, S.M., Dykes, G.A., Rahman, S. (2017).Draft Genome Sequences of Six Enterococcus faecalis Strains Isolated from Malaysian Clinical and Environmental Origins,, Genome Announcements, Vol. 5, No. 24. Daniel, D.S., Lee, S.M., Gan, H.M., Dykes, G.A., Rahman, S.(2017) )Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia, . Journal of Infection and Public Health Vol. 10, No. 5, 617-623.Elmali, M., Can, H.Y. (2017). Occurence and antimicrobial resistance of Arcobacter species in food and slaughterhouse samples, , Journal of Food Science and Technology, Vol. 37, No. 2, 280-285 (2017). Fibriana, F., Amalia, A.V., Mubarok,I. (2017). )Isolation and Characterization of Pigment Generating Microorganisms from Potato Skin. . Jurnal Mipa, Vol. 40, No. 1, 7-13 (2017).Guran, H.S., Vural, A., Erkan, M.E., Durmusoglu. (2017)Prevalence and some virulence genes of E. coli O157 isolated from chicken meats and giblets Ann. Anim. Sci., Vol. 17, No. 2 (2017) 555–563 Idrees, H.A., Omer, A.F.A., Elaas, W.A., Mohammed, Z.M.S., Sidig, M., (2017) Serological Significance of Anti-HBc IgG Marker detected in Sudanese Haemodialysis Patients . American Journal of Research Communication2017, 5(8): 1-8.Wilantho, A., Deekaew, P., Srissutti, C., Tongisma, S., Somboonna, N. (2017). Diversity of bacterial communities on the facial skin of different age-group Thai males PeerJ, (2017).Zakaria, N.H., Tajarudin, H.A., Sapingi, M.S.M., Murshed, M.F. (2017).Identification of Pathogenic Bacteria Isolated from Raw and After Sand Filtration Water at Lubok Buntar Water Treatment Plant, Scientific Research Journal, Vol. 14, No. 1 (2017).Azwai, S.M.et al. (2016) Isolation and Molecular Identification of Vibrio spp. By sequencing of 16S rDNA from seafood, meat and meat products in Libya. Open Veterinary Journal, 6(1), p. 36-43.Aw, Y.K., et al (2016) Newly Isolated Paenibacillus tyrfis sp. nov., from Malaysian Tropical Peat Swamp Soil with Broad Spectrum Antimicrobial Activity. Frontiers in Microbiology. 7(219), p. 1-9.Copur-Cicek,A., Dinc-Patlak, R., Sandalli, C., Mengeloğlu, Z., Kostakoglu, U., Yildiz, I.E. (2016). Determination of the staphylococcal cassette chromosome in methicillin-resistant Staphylococcus aureus strains isolated from various clinical samples. . Russia Open Medical Journal. 5(2). Luang-In,V., Deeseenthum, S. (2016) Exopolysaccharide-producing isolates from Thai milk kefir and their antioxidant activities. . LWT - Food Science and Technology.73. Pp592-601 Merghani, E.A.E., Elsanousi, S.M., Hamedelnil, Y.F., Gornas, N.A. (2016).Molecular Study of Bacterial Pathogens Isolated From Haemodialysis Patients, Sudan Journal of Science and Technology, Vol 12, No. 1, 1-10 (2016).Mohamad, Y., Reda, W.W., Abdel-Moein, K., El-Razil, K.A.A., Barakat, A.M.A., El Fadaly, H.A., Hassanain, N.A., Hegazi, A.G. (2016) Prevalence and phylogenetic characterization of Listeria Monocytogenes isolated from processed meat marketed in Egypt.. Journal of Genetic Engineering and Biotechnology. 14(1). Pp.119-123 Raweekul, W., Wuttitummaporn, S., Sodchuen, W., Kittiwongwattana, C. (2016).Plant Growth Promotion by Endophytic Bacteria Isolated from Rice (Oryza sativa), Thammasat International Journal of Science and Technology,Vol. 21, No. 1 (2016).Supong, K., Thawai, C., Supothina, S., Auncharoen, P., Pittayakhajonwut, P. (2016) ) Antimicrobial and anti-oxidant activities of quinoline alkaloids from Pseudomonas aeruginosa BCC76810. Phytochemistry Letters. 17. Pp.100-106Uttatree,S., Charoenpanich, J. (2016) Isolation and characterization of a broad pH- and temperature-active, solvent and surfactant stable protease from a new strain of Bacillus subtilis. . Biocatalysis and Agricultural Biotechnology. 8. Pp.32-38Abdulrahman, S.M. (2015). Multiplex PCR for direct identification of Campylobacter Species in Human Stool Journal of Biomedical and Pharmaceutical Research, Vol. 4, No. 3 (2015). Akinsaya, M. A. et al. (2015) Metagenomics Study of Endophytic Bacteria in Aloe Vera Using Next Generation Technology. Genomics Data. ScienceDirect6, p. 159-163. Chua, P., Har, Z.M., Austin, C.M., Yule, C.N., Dykes, G.A., Lee, S.M. (2015) Genome sequencing and annotation of Aeromonas sp. HZ. . Genomics Data. 5 pp38-39 Chua, P., Har, Z.M., Austin, C.M., Yule, C.N., Dykes, G.A., Lee, S.M. (2015) Genome sequencing and annotation of Cellulomonas sp. HZM. . Genomics Data.5. Pp40-41Erdogmus, S.F., et al (2015) Aromatic Hydrocarbon Utilization Ability of Chromohalobacter sp. Ekoloji94: 10-16. Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015)Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels. Virology Journal. 12(102), p. 1-10.Ntougias, S., Melidis, P., Navrozidou, E., Tzegkas, F. (2015) Diversity and efficiency of anthracene-degrading bacteria isolated from a denitrifying activated sludge system treating municipal wastewater. International Biodeterioration & Biodegradation. 97. Pp..151-158.Abd El-Salam, N.M., Ayaz, S., & Ullah, R. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa. BioMed Research International. Abd El-Salam, N.M., Ayaz, S., & Ullah, R. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa. BioMed Research International. Chua, P., et al. (2014)Draft Genone Sequences of Two Cellulolytic Paenibacillus sp. Strains, MAEPY1 and MAEPY2, from Malaysian Landfill Leachate. Genone Announcements.2(1). El-Salam et al. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa Biomed Res Int92014: 861831. Heng, J.L.S., & Hamzah, H. (2014) Biological Active Compounds from Actinomycetes Isolated from Soil of Langkawi Island, Malaysia. African Journal of Biotechnology.ProQuest. 13(49), p. 4523-4528 Rahama, A.B.M., et. al (2014) Molecular Detection of helicobacter pylori Among Gastroduodenitis and Peptic Ulcer Patients in Khartoum State. Journal of Biomedical and Pharmaceutical Research3(5): 41-44 Ting, A.S.Y., Hermanto, A., Peh, K.L. (2014)Indigenous actinomycetes from empty fruit bunch compost of oil palm: Evaluation on enzymatic and antagonistic properties. Biocatalysis and Agricultural Biotechnology. 3. Pp..310-315.Wanangkarn, A., Liu, D.C., Swetwiwathana, A., Jindaprasert, A., Phraephaisarn, C., Chumnqoen, W., Tan, F.J. (2014) Lactic acid bacterial population dynamics during fermentation and storage of Thai fermented sausage according to restriction fragment length polymorphism analysis. International Journal of Food Microbiology. 186. Pp..61-67Erdomus, S.F., et al. (2013) Aromatic Hydrocarbon Degradation by Halophilic Archaea Isolated from Camalti Saltern, Turkey Water, Air and Soil Pollution. ProQuest. p. 1-9.Hafshejani, M.K., et al (2013)Sequential Microaerophilic-Oxic Phase Mineralization of Azo Dyes by a Monoculture of Pseudomonas Aeruginosa Strain AWF Isolated from Textile Wastewater Water Air and Soil Pollution. ProQuest. p. 1-16.Naher, U.A., Othman, R., Abdul-Latif, M., Panhwar, Q.A., Amaddin, P.A.M., Shamsuddin, Z.H. (2013). Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia, International Journal of Molecular Sciences, Vol. 14, 17812-17829 (2013).Gumel et al.(2012)Biosynthesis and Characterization of Polyhydroxyalkanoates Copolymers Produced by Pseudomonas putida Bet001 Isolated from Palm Oil Mill Effluent PLoS One7(9).Pongsilp, N., et al (2012) Genotypic Diversity among Rhizospheric Bacteria of Three Legumes Assessed by Cultivation-dependent and Cultivation-independent Techniques World Journal of Microbiology and Biotechnology. ProQuest. 28, p. 615-626.Shamsudin, M.N., et al (2012)High Prevalence of qacA/B Carriage among Clinical Isolates of Meticillin-resistant Staphylococcus aureus in Malaysia Journal of Hospital Infection81: 206Charoenpanich, J., Suktanarag, S., & Toobbucha, N. (2011)Production of Thermostable Lipase by Aeromonas sp. EBB-1 Isolated from Marine Sludge in Angsila, Thailand ScienceAsia37: 105-114.Mat Hussin, N. H. et al (2011)Characterization of Bacteria and Fungi Bioaerosol in the Indoor Air of Selected Primary Schools in Malaysia Indoor and Built Environment. Sage Journals20(6), p. 607-617Atshan, S.S., & Shamsudin, M.N. (2010)Evaluation of Phenotypic and Genotypic Detection Methods for Biofilm-forming Methicillin-sensitive and Methicillin-resistant Staphylococcus aureus Clinical Isolates Annals of Microbiology. Uttatree, S., Winayanuwattikun, P., & Charoenpanich, J. (2010) Isolation and Characterization of a Novel Thermophilic-organic Solvent Stable Lipase from Acinetobacter baylyi Applied Biochemistry and Biotechnology Zakaria, M.R., et al. (2010)Polyhydroxyalkanoate Production from Anaerobically Treated Palm Oil Mill Effluent by New Bacterial Strain Comamonas sp. EB172 World Journal of Microbiology and Biotechnology. ProQuest. 26, p. 767-774.

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。