DescriptionThe GF-1 AmbiClean Kit (Gel & PCR) is designed for rapid DNA recovery from agarose gel and PCR clean-up of DNA bands ranging from 100bp to 20kb. Special buffer provide the correct salt concentration and pH for efficient recovery (80-90%) of DNA from both PCR product and agarose gel from TAE or TBE buffers. The kit is well suited for the removal of agarose, excess dNTPs, short oligo fragments, mineral oil, enzymes from a PCR reaction product, proteins after restriction enzyme treatment and dephosphorylation, residual dye and ethidium bromide. This kit also allows for concentration of DNA, changing of buffers and desalting.

Features

• 90% of recovery achievable
• Purification process less than 15 minutes
• High pure DNA ready-to-use for routine molecular biology applications such as restriction enzyme digestion, PCR, ligation, DNA sequencing, probe preparations, etc.

Kit Components

• Buffer DB
• Wash Buffer (concentrate)
• Elution Buffer

Ordering Information

Catalog No	Description	Pack Size
GF-GC-050	GF-1 AmbiClean Kit (Gel & PCR)	50 preps
GF-GC-100	GF-1 AmbiClean Kit (Gel & PCR)	100 preps
GF-GC-200	GF-1 AmbiClean Kit (Gel & PCR)	200 preps

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GF-1 AmbiClean Kit (Gel & PCR)

PublicationThis Product Has Been Used In: Al-Jallad et al. (2020)Molecular Characterization of Isolated Infectious Bronchitis Viruses from Affected Vaccinated Broiler Flocks in Syria, BMC Veterinary Research, 16:449.Bacus, MG et al. (2020) Global genetic patterns reveal host tropism versus cross-taxon 1 transmission of bat Betacoronaviruses, bioRxiv, https://doi.org/10.1101/2020.05.04.076281Donhatai Sruamsiri et al. (2020). In situ identification of polyhydroxyalkanoate (PHA)-accumulating microorganisms in mixed microbial cultures under feast famine conditions,. Scientific Reports,10:3752.Ekprasert J et al. (2020) Investigating mechanical properties and biocement application of CaCO3, precipitated by a newly-isolated Lysinibacillus sp. WH using artificial neural networks, Scientific Reports,10:16137.Eshamah HL et al. (2020)Extent of pathogenic and spoilage microorganisms in whole muscle meat, meat products and seafood sold in Libyan market, Open Veterinary Journal,10(3):276-288.Elv Nhiel Salo & Annabelle Novero (2020) Identification and Characterisation of Endophytic Bacteria from Coconut (Cocos nucifera) Tissue Culture, Tropical Life Sciences Research,31(1), 2020.Insuk C, Kuncharoen N, Cheeptham N, Tanasupawat S & Pathom-aree W (2020)Bryophytes Harbor Cultivable Actinobacteria With Plant Growth Promoting Potential, Frontiers in Microbiology, 11:563047.Pimsiri Heepkaew & Benjaporn BS (2020) Polyhydroxyalkanoate production using two stage continuous stirred tank activated sludge systems with glycerol as a carbon source, Journal of Chemical Technology & Biotechnology, 95:1180-1190.Rahnama R et al. (2020)Definite diagnosis of viral haemorrhagic septicaemia infected farmed rainbow trout and histopathologic study of acutely diseased fish in Iran, Iranian Journal of Fisheries Sciences, 19(3):1556-1572.Tamosiunalte A et al. (2020) What a Difference a Gene Makes: Identification of Virulence Factors of Cowpox Virus, Journal of Virology, 94:e01625-19.Luang-In et al. (2019) Protease-Producing Bacteria from Soil in Nasinuan Community Forest, Mahasarakham Province, Thailand, Biomedical and Pharmacology Journal, 12(2):587-595 Chong, S.Y., Rao, P.V. and Soon, Jan Mei (2017)Identification of Escherichia spp. strains in streetvended beverages and associated preparation surfaces using 16S rRNA analysis. International Food Research Journal, 24 (4). pp. 18111818Garbaj, A.M., Abolghait, S.K., Lawila, A.F., Azwai, S.M., Naas, H.T., Moawad, A.A., Gammoudi, F.T., Barbieri, I., Abureema, S., Eldaghayes, I. (2017). Molecular Identification, Prevalence and Antimicrobial Susceptibility Profile of Cronobacter spp. Cultivated on a Chromogenic Medium in Libya,Journal of Molecular Microbiology, Vol. 1, No. 1 (2017).Pasandideh, R., Nassiri, B., Shapouri, M.R.S.A., Fayazi, J., Roshanfeker, H., Lotfi, M (2017).Expression of the G1 Epitope of Bovine Ephemeral Fever Virus G Glycoprotein in Eukaryotic cells, Bulgarian Journal of Veterinary Medicine, 1311-1477 (2017). Said, M.B., Belkahia, H., Mabrouk,N.E., Saidani, M., Hassen, M.B., Alberti, A., Zobba, R., Bouattour, S., Bouattour, A., Messadi, L. (2017)Molecular typing and diagnosis of Anaplasma spp. closely related to Anaplasma phagocytophilum in ruminants from Tunisia. Ticks and Tick-borne Diseases.8. Pp..412-422Said, M.B., Belhakia, H., Mabrouk, N.E., Saidani, M., Alberti ,A., Zobba,R., Cherif, A., Mahjoub, T., Bouattour, A., Messadi, L. (2017)Anaplasma platys-like strains in ruminants from Tunisia. Infection, Genetics and Evolution 49. Pp..226-233.Shamsi, H., Mardani, K., Ownagh, A. (2017). Phylogenetic analysis of Escherichia coli isolated from broilers with colibacillosis based on gyrA gene sequences. The Canadian Journal of Veterinary Research, Vol (81), 28-32 (2017).Azwai, S.M.et al. (2016) Isolation and Molecular Identification of Vibrio spp. By sequencing of 16S rDNA from seafood, meat and meat products in Libya Open Veterinary Journal, 6(1), p. 36-43. Sriwichai, M., Malem, F., Pholchan, M.K., Bovonsombut, S. (2017).Detection of Bacterial Communities in Volatile-organic-compound (VOC)-contaminated Soil in an Industrial Estate in Eastern Thailand by PCR-DGGE Analysis, Chiang Mai Journal of Science, Vol. 44, No. 3, 742-750 (2017).Abadi, Z.S.N., Fatemi, F., Khosravani, M., Rahmati, M., Sadroddiny, E (2015).Molecular Cloning of Bone Morphogenetic Protein-2 (BMP- 2) in pGEM-b1 Vector and Transformation into E.coli Rosetta Strain, International Journal of Health Sciences, Vol. 1, No. 3 (2015).Belkahia, H., Said, M.B., Alberti, A., Abdi, K., Issaoui, Z., Hattab, D., Ghabi, M., Messadi, L. (2015) First molecular survey and novel genetic variants’ identification of Anaplasma marginale, A. centrale and A. bovis in cattle from Tunisia. Infection, Genetics and Evolution. 34. Pp.361-371 Belkahia, H., Said, M.B., Sayahi, L., Alberti, A., Messadi, L. (2015). Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius), The Journal of Infection in Developing Countries, Vol. 9, No. 10, 1116-1125 Barghi, N., Concepcion, G. P., Olivera, B. M., Lluisma, A.O. (2015). Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes, Genome Biology and Evolution, Vol. 7, No. 6, 1797-1814 (2015).Deesuth, O., Laopaiboon, P., Klanrit, P., Laopaiboon, L. (2015) Improvement of ethanol production from sweet sorghum juice under high gravity and very high gravity conditions: Effects of nutrient supplementation and aeration. Industrial Crops and Products. 74. Pp.95-102.Emine, S., Zainol, N., Salihon, J., Convey, P (2015). Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi, International Journal of Extensive Research, No. 5, 79-87 (2015). Hamidinejat, H., et al. (2015)Development of an Indirect ELIS using Different Fragments of Recombinant Ncgra 7 for Detection of Neospora caninum Infection in Cattle and Water Buffalo. OIran Journal of Parasitology. 10(1), p.69-77. Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015)Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels. Virology Journal. 12(102), p. 1-10.Nasanit, R., et al (2015) Assesment of Epiphytic Yeast Diversity in Rice (Oryza sativa) Phyllospehere in Thailand by a Culture-independent Approach Antonie van Leeuwenhoek.Springer. 107(6), p.1475-1490 Said, M.B., Belkahia, H., Karaoud, M., Bousrih, M., Yahiaoui, M. Daaloul-Jedidi, M., Messadi, L. (2015) First molecular survey of Anaplasma bovis in small ruminants from Tunisia.Veterinary Microbiology. 179. Pp.322-326.Seydametov, E (2015).Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil, International Journal of Bioscience, Biochemistry and Bioinformatics, Vol. 6, No. 2 (2015). Wiparat,S., Poeaim, S., Eiamampai, K., Atittayawan (2015). Gender Identification of Himantopus Himantopus Using PCR-Based Method, International Journal of Agricultural Technology, Chiang Mai Journal of Science, Vol. 11, No.2, 307-314 (2015).Belkahia, H., Said, M.B., Hamdi, S.E., Yahiaoui, M., Gharbi, M., Daaloul-Jedidi, M., Mhadbi, M., Jedidi, M., Darghout, M.A., Klabi, I., Zribi, L., Messadi, L. (2014)) First molecular identification and genetic characterization ofAnaplasma ovis in sheep from Tunisia. Small Ruminant Research. 121. Pp.404-410. Mahboubi, M., Falsafi, T., Sadeghizadeh, M (2014). Cloning and Sequence Analysis of Gene Encoding OipA from Iranian Clinical Helicobacter pylori, Iranian Journal of Biotechnology, Vol 12, No. 4, 10-16 (2014).Şakalar, Ç., Kuk, S., Erensoy, A., Dağli, A.F., Özercan, İ., Çetikaya, Ü, Yazar, S. (2014). Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR- RFLP method with the potential use for other Echinococcus species, Turkish Journal of Medical Sciences, Vol. 44, 741-748 (2014). Xu, Z., Zikos, D., Tamošiunaite, A., Klopfleisch, R., Osterrieder, N., Tischer, B.K. (2014).Identification of 10 Coxpox Virus Proteins that are necessary for Induction of Hemorrhagic Lesions (Red Pocks) on Chorioallantoic Membranes,Vol. 88, No. 15 (2014). Nayabian, H., Mardani, K. (2013).Molecular characterization of the chicken anaemia viruses isolated from broiler farms of west Azerbaijan, Iran, Avian Pathology, Vol. 42, No. 2, 108-113 (2013). Supathaweewat, K., Klanrit, P (2013). cDNA cloning and expression analyses of phytoene synthase 1, phytoene desaturase and -carotene desaturase genes from Solanum lycopersicum KKU-T34003, Songklankarin Journal of Science and Technology, Vol. 35, No. 5, 517-527 (2013).

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。