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蚂蚁淘在线 / 品牌中心 / RayBiotech / RayBiotech/Human JAK/STAT Pathway Phosphorylation Array C1/2 Sample Kit/AAH-JAKSTAT-1-2
商品描述
Antigen Information
Gene Symbols:
EGFR | JAK1 | JAK2 | PTPN11 | PTPN6 | SRC | STAT1 | STAT2 | STAT3 | STAT5A| STAT6 | TYK2 View more Hide
Assay Format
Pathway:
- Akt Signaling
- HER/ErbB Signaling
- JAK/STAT Signaling
- MAPK Signaling
- Notch Signaling
- PI3K-AKT Signaling
Number of Targets Detected:
12
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
EGFR (P-Ser1070)
| JAK1 (P-Tyr1022)
| JAK2 (P-Tyr1007/1008)
| SHP-1 (P-Ser591)
| SHP2 (P-Tyr542)
|
Src (P-Tyr419)
| STAT1 (P-Ser727)
| STAT2 (P-Tyr689)
| STAT3 (P-Tyr705)
| STAT5 (P-Tyr694)
|
STAT6 (P-Tyr641)
| TYK2 (P-Tyr1054)
| |||
Typical Results
Figure 1: A431 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with EGF for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human JAK/STAT Pathway Phosporylation Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP"s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Zheng, Shutao, et al. "NME4 modulates PD-L1 expression via the STAT3 signaling pathway in squamous cell carcinoma."?Biochemical and Biophysical Research Communications?(2020).Species: HumanSample type: Cell Lysate
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Manual
SDS
Analysis Tool
