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蚂蚁淘在线 / 品牌中心 / RayBiotech / RayBiotech/Mouse Apoptosis Signaling Pathway Array C1/2 Sample Kit/AAM-APOSIG-1-2

RayBiotech/Mouse Apoptosis Signaling Pathway Array C1/2 Sample Kit/AAM-APOSIG-1-2

价格
面议
货号：AAM-APOSIG-1-2
浏览量：127
品牌：RayBiotech
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商品描述

Antigen Information

Gene Symbols:
AKT1 | ATM | BAD | CASP3 | CASP7 | CDKN1B | CHEK1 | EIF2S1 | HSPB1 | MAP3K7 View more Hide

Assay Format

Pathway:
  • Akt Signaling
  • HER/ErbB Signaling
  • HIF-1 alpha Signaling
  • IGF Signaling
  • JAK/STAT Signaling
  • MAPK Signaling
  • mTOR Signaling
  • NFkB Signaling
  • p53 Signaling
  • PI3K-AKT Signaling
  • PKC Signaling
  • TGF-beta Signaling
Number of Targets Detected:
17
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
AKT (P-Ser473)
  • Gene Symbol: Akt1 Akt Rac
  • Gene ID: 11651
  • Accession #: P31750
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA
ATM (P-Ser1981)
  • Gene Symbol: Atm
  • Gene ID: 11920
  • Accession #: Q62388
  • ELISA Kit: RayBio Human Phospho-ATM (S1981) ELISA
BAD (P-Ser112)
  • Gene Symbol: Bad Bbc6
  • Gene ID: 12015
  • Accession #: Q61337
  • ELISA Kit: RayBio Human Phospho-BAD (S112) ELISA
Caspase-3 (P-Asp175)
  • Gene Symbol: Casp3 Cpp32
  • Gene ID: 12367
  • Accession #: P70677
  • ELISA Kit: RayBio Human Cleaved-Caspase-3 (D175) and Caspase-3 ELISA
Caspase-7 (P-Asp198)
  • Gene Symbol: Casp7 Lice2 Mch3
  • Gene ID: 12369
  • Accession #: P97864
  • ELISA Kit: RayBio Human/Mouse Cleaved-Caspase-7 (D198) and Caspase-7 ELISA
CHK1 (P-Ser296)
  • Gene Symbol: Chek1 Chk1
  • Gene ID: 12649
  • Accession #: O35280
  • ELISA Kit: RayBio Human Phospho-Chk1 (S280) ELISA
eIF-2a (P-Ser52)
  • Gene Symbol: Eif2s1 Eif2a
  • Gene ID: 13665
  • Accession #: Q6ZWX6
  • ELISA Kit: RayBio Human Phospho-eIF-2A (S52) ELISA
ERK1/2 (P-Thr202)
  • Accession #: Q63844 | P63085
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) ELISA
HSP27 (P-Ser82)
  • Gene Symbol: Hspb1 Hsp25 Hsp27
  • Gene ID: 15507
  • Accession #: P14602
  • ELISA Kit: RayBio Human Phospho-HSP27 (S82) ELISA
IKBa (P-Ser32)
  • Gene Symbol: Nfkbia Ikba
  • Gene ID: 18035
  • Accession #: Q9Z1E3
  • ELISA Kit: RayBio Human Phospho-I-kappa B alpha (S32) ELISA
JNK (P-Thr183)
  • Gene Symbol: Mapk8 Jnk1 Prkm8
  • Gene ID: 26419
  • Accession #: Q91Y86
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA
NF-kB P65 (P-Ser536)
  • Gene Symbol: Rela Nfkb3
  • Gene ID: 19697
  • Accession #: Q04207
  • ELISA Kit: RayBio Human Phospho-NF-KB p65 (S536) ELISA
p27 (P-Thr198)
  • Gene Symbol: Cdkn1b
  • Gene ID: 12576
  • Accession #: P46414
  • ELISA Kit: RayBio Human Phospho-P27/Kip1 (T198) ELISA
p38 (P-Thr180/Tyr182)
  • Gene Symbol: Mapk14 Crk1 Csbp1 Csbp2
  • Gene ID: 26416
  • Accession #: P47811
  • ELISA Kit: RayBio Human/Mouse Phospho-p38 alpha (T180/Y182) ELISA
P53 (P-Ser15)
  • Gene Symbol: Tp53 P53 Trp53
  • Gene ID: 22059
  • Accession #: P02340
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA
SMAD2 (P-Ser245)
  • Gene Symbol: Smad2 Madh2 Madr2
  • Gene ID: 17126
  • Accession #: Q62432
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) ELISA
TAK1 (P-Ser412)
  • Gene Symbol: Map3k7 Tak1
  • Gene ID: 26409;
  • Accession #: Q62073
  • ELISA Kit: RayBio Human Phospho-TAK1 (Ser412) ELISA
   

Typical Results

Figure 1: NIH/3T3 cells were grown to 80% confluency and then serum starved overnight. Cells were treated (left panel) with Camptothecin (CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Apoptosis Signaling Pathway Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP"s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Radulovic, Katarina, et al. "NLRP6 Deficiency in CD4 T Cells Decreases T Cell Survival Associated with Increased Cell Death."?The Journal of Immunology?203.2 (2019): 544-556.Species: MouseSample type: Cell Lysates
  2. Liu F, Qiu H, Xue M, et al. MSC-secreted TGF-? regulates lipopolysaccharide-stimulated macrophage M2-like polarization via the Akt/FoxO1 pathway.?Stem Cell Res Ther. 2019;10(1):345. Published 2019 Nov 26. doi:10.1186/s13287-019-1447-ySpecies: MouseSample type: Lysates
It is important to select the protein concentration as a positive sample before the experiment. I recommend that western blot or another experiment should be confirmed an expression level before using a kit. Optionally, for a clear image under the low background, I recommend using a high sensitivity ECL solution.
Hyuk-Kwon KwonYale University06/28/2019, 05:19 PM
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#Manual

#SDS

#Analysis Tool

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