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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
CCL2 | CCL5 | CSF1 | CSF2 | CXCL1 | IFNG | IL10 | IL12A | IL13 | IL17A| IL1A | IL1B | IL2 | IL3 | IL4 | IL5 | IL6 | IL9 | TNF | VEGFA View more Hide
Assay Format
Pathway:
- MAPK Signaling
Number of Targets Detected:
20
Species Detected:
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 alpha(IL-1 F1)
| IL-1 beta(IL-1 F2)
| IL-10
|
IL-12 p70
| IL-13
| IL-17A
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-9
| KC(CXCL1)
|
MCP-1(CCL2)
| M-CSF
| RANTES(CCL5)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Fink DM, Connor AL, Kelley PM, Steele MM, Hollingsworth MA, Tempero RM. Nerve Growth Factor Regulates Neurolymphatic Remodeling during Corneal Inflammation and Resolution. Ng LG, ed. PLoS ONE 2014;9(11):e112737. doi:10.1371/journal.pone.0112737.Species: MouseSample type: Tissue Lysate
- Huang X, Ren L, Ye P, Cheng C, Wu J, et al. (2014) Peroxisome Proliferator Activated Receptor c Deficiency in T Cells Accelerates Chronic Rejection by Influencing the Differentiation of CD4+ T Cells and Alternatively Activated Macrophages. PLoS ONE 9(11): e112953. doi:10.1371/journal.pone.0112953Species: MouseSample type: Conditioned Media
- Karra L., Haworth O., Priluck R., et al. Lipoxin B4 promotes the resolution of allergic inflammation in the upper and lower airways of mice. Mucosal Immunol. 2014 Dec 3. doi: 10.1038/mi.2014.116.Species: MouseSample type: Serum
- Guo H., Yun C., Hou G., Du J., Huang X., et al. Mangiferin Attenuates Th1/Th2 Cytokine Imbalance in an Ovalbumin-Induced Asthmatic Mouse Model. PLOS One. Published: June 23, 2014. DOI: 10.1371/journal.pone.0100394Species: MouseSample type: Serum
- Zingariello, Maria, et al. "Characterization of the TGF-?1 signaling abnormalities in the Gata1 low mouse model of myelofibrosis."?Blood?121.17 (2013): 3345-3363.Species: MouseSample type: Conditioned Media
- Arevalo-Martin A., Molina-Holgado E., Guaza C. A CB?/CB? receptor agonist, WIN 55,212-2, exerts its therapeutic effect in a viral autoimmune model of multiple sclerosis by restoring self-tolerance to myelin. Neuropharmacology. 2012 Sep;63(3):385-93. doi: 10.1016/j.neuropharm.2012.04.012. Species: MouseSample type: Tissue Lysate
- Robbins D., Li W., Humphrey K., Zhao Y. Redox Factor-1 Mediates Inflammatory Response during Tumor Promotion in Skin Epidermal JB6 Cells. Open Journal of Apoptosis, 2012, 1, 19-24. http://dx.doi.org/10.4236/ojapo.2012.13003Species: MouseSample type: Cell Lysate
- Vila` L, Roglans N, Baena M, Barroso E, Alegret M, et al. (2012) Metabolic Alterations and Increased Liver mTOR Expression Precede the Development of Autoimmune Disease in a Murine Model of Lupus Erythematosus. PLoS ONE 7(12): e51118. doi:10.1371/journal.pone.0051118Species: MouseSample type: Conditioned Media
- Kelley P., et al. Regressed lymphatic vessels develop during corneal repair. Lab Invest. 2011 Nov;91(11):1643-51. doi: 10.1038/labinvest.2011.121.Species: MouseSample type: Tissue Lysate
- Ojeda, Gloria, et al. "Complement regulatory protein Crry/p65 costimulation expands natural Treg cells with enhanced suppressive properties in proteoglycan?induced arthritis."?Arthritis & Rheumatism?63.6 (2011): 1562-1572.Species: MouseSample type: Conditioned Media
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