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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
AREG | BDNF | BMP4 | BMP5 | BMP7 | CCL1 | CCL11 | CCL15 | CCL2 | CCL24| CCL3 | CCL4 | CCL5 | CSF1 | CSF1R | CSF2 | CSF3 | CXCL13 | CXCL8 | CXCL9 | EGF | EGFR | FGF2 | FGF4 | FGF7 | FIGF | FLT4 | GDF15 | GDNF | GH1 | HBEGF | HGF | ICAM1 | IFNG | IGF1 | IGFBP1 | IGFBP2 | IGFBP3 | IGFBP4 | IGFBP6 | IL10 | IL11 | IL12A | IL12B | IL13 | IL15 | IL16 | IL17A | IL1A | IL1B | IL1RN | IL2 | IL4 | IL5 | IL6 | IL6R | IL7 | INS | KDR | KIT | KITLG | LTA | NGF | NGFR | NTF3 | NTF4 | PDGFA | PDGFB | PGF | PROK1 | TGFA | TGFB1 | TGFB3 | TIMP1 | TIMP2 | TNF | TNFRSF11B | TNFRSF1A | TNFRSF1B | VEGFA View more Hide
Assay Format
Number of Targets Detected:
80
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 100 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Amphiregulin
| BDNF
| beta-NGF
| bFGF
| BLC(CXCL13)
|
BMP-4
| BMP-5
| BMP-7
| EGF
| EGFR
|
Eotaxin-1(CCL11)
| Eotaxin-2(MPIF-2/CCL24)
| FGF-4
| FGF-7(KGF)
| GCSF
|
GDF-15
| GDNF
| GM-CSF
| Growth Hormone
| EG-VEGF(PK1)
|
HB-EGF
| HGF
| I-309(TCA-3/CCL1)
| ICAM-1(CD54)
| IFN-gamma
|
IGF-1
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
IGFBP-6
| IL-1 alpha(IL-1 F1)
| IL-1 beta(IL-1 F2)
| IL-1 ra(IL-1 F3)
| IL-10
|
IL-11
| IL-12 p40
| IL-12 p70
| IL-13
| IL-15
|
IL-16
| IL-17A
| IL-2
| IL-4
| IL-5
|
IL-6
| IL-6 R
| IL-7
| IL-8(CXCL8)
| Insulin
|
MCP-1(CCL2)
| M-CSF
| M-CSF R
| MIG(CXCL9)
| MIP-1 alpha(CCL3)
|
MIP-1 beta(CCL4)
| MIP-1 delta(CCL15)
| NGFR(TNFRSF16)
| NT-3
| NT-4
|
Osteoprotegerin(TNFRSF11B)
| PDGF-AA
| PDGF-BB
| PLGF
| RANTES(CCL5)
|
SCF
| SCF R(CD117/c-kit)
| TGF alpha
| TGF beta 1
| TGF beta 3
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta(TNFSF1B)
| TNF RI(TNFRSF1A)
|
TNF RII(TNFRSF1B)
| VEGF-A
| VEGF-D
| VEGFR2
| VEGFR3
|
Species Detected
Human
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Human Cytokine Array Q1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Massee M., Chinn K., Lim J., et al. Type I and II Diabetic Adipose-Derived Stem Cells Respond In Vitro to Dehydrated Human Amnion/Chorion Membrane Allograft Treatment by Increasing Proliferation, Migration, and Altering Cytokine Secretion. Adv Wound Care (New Rochelle). 2016 Feb 1;5(2):43-54 Species: HumanSample type: Conditioned Media
- Ciavarella S., Caselli A., Tamma A., et al. A peculiar molecular profile of umbilical cord-mesenchymal stromal cells drives their inhibitory effects on multiple myeloma cell growth and tumor progression. Stem Cells Dev. 2015 Jun 15;24(12):1457-70. doi: 10.1089/scd.2014.0254. Species: HumanSample type: Conditioned Media
- Massee M., Chinn K., Lei J., et al. Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro. J Biomed Mater Res B Appl Biomater. 2015 Jul 14. doi: 10.1002/jbm.b.33478Species: HumanSample type: Conditioned Media
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Manual
SDS
Analysis Tool (QAH-INF-3)
Analysis Tool (QAH-GF-1)
