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RayBiotech/Mouse Cytokine Array Q1/8 Sample Kit/QAM-CYT-1-1

价格
面议
货号:QAM-CYT-1-1
浏览量:127
品牌:RayBiotech
服务
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商品描述

Antigen Information

Gene Symbols:
CCL2 | CCL5 | CSF1 | CSF2 | CXCL1 | IFNG | IL10 | IL12A | IL13 | IL17A View more Hide

Assay Format

Pathway:
  • MAPK Signaling
Number of Targets Detected:
20
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF
  • Gene Symbol: CSF2
  • Accession #: P26955
  • ELISA Kit: RayBio Mouse GM-CSF ELISA
IFN-gamma
  • Gene Symbol: IFNG
  • Accession #: P01580
  • ELISA Kit: RayBio Mouse IFN-gamma ELISA
IL-1 alpha(IL-1 F1)
  • Gene Symbol: IL1A
  • Accession #: P01582
  • ELISA Kit: RayBio Mouse IL-1 alpha ELISA
IL-1 beta(IL-1 F2)
  • Gene Symbol: IL1B
  • Accession #: P10749
  • ELISA Kit: RayBio Mouse IL-1 beta ELISA
IL-10
  • Gene Symbol: IL10
  • Accession #: Q0VBJ1
  • ELISA Kit: RayBio Mouse IL-10 ELISA
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
  • Gene Symbol: IL13
  • Accession #: P20109
  • ELISA Kit: RayBio Mouse IL-13 ELISA
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
  • ELISA Kit: RayBio Mouse IL-17A ELISA
IL-2
  • Gene Symbol: IL2
  • Accession #: Q791T3
  • ELISA Kit: RayBio Mouse IL-2 ELISA
IL-3
  • Gene Symbol: IL3
  • Accession #: P01586
  • ELISA Kit: RayBio Mouse IL-3 ELISA
IL-4
  • Gene Symbol: IL4
  • Accession #: P07750
  • ELISA Kit: RayBio Mouse IL-4 ELISA
IL-5
  • Gene Symbol: IL5
  • Accession #: P04401
  • ELISA Kit: RayBio Mouse IL-5 ELISA
IL-6
  • Gene Symbol: IL6
  • Accession #: P08505
  • ELISA Kit: RayBio Mouse IL-6 ELISA
IL-9
  • Gene Symbol: IL9
  • Accession #: P15247
  • ELISA Kit: RayBio Mouse IL-9 ELISA
KC(CXCL1)
  • Gene Symbol: CXCL1
  • Accession #: P12850
  • ELISA Kit: RayBio Mouse KC ELISA
MCP-1(CCL2)
  • Gene Symbol: CCL2
  • Accession #: Q9QYD7
  • ELISA Kit: RayBio Mouse MCP-1 ELISA
M-CSF
  • Gene Symbol: CSF1
  • Accession #: P07141
  • ELISA Kit: RayBio Mouse M-CSF ELISA
RANTES(CCL5)
  • Gene Symbol: CCL5
  • Accession #: P30882
  • ELISA Kit: RayBio Mouse RANTES ELISA
TNF alpha
  • Gene Symbol: TNF
  • Accession #: Q62326
  • ELISA Kit: RayBio Mouse TNF-alpha ELISA
VEGF-A
  • Gene Symbol: VEGFA
  • Accession #: Q00731
  • ELISA Kit: RayBio Mouse VEGF-A ELISA

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Mouse Cytokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Fink DM, Connor AL, Kelley PM, Steele MM, Hollingsworth MA, Tempero RM. Nerve Growth Factor Regulates Neurolymphatic Remodeling during Corneal Inflammation and Resolution. Ng LG, ed. PLoS ONE 2014;9(11):e112737. doi:10.1371/journal.pone.0112737.Species: MouseSample type: Tissue Lysate
  2. Huang X, Ren L, Ye P, Cheng C, Wu J, et al. (2014) Peroxisome Proliferator Activated Receptor c Deficiency in T Cells Accelerates Chronic Rejection by Influencing the Differentiation of CD4+ T Cells and Alternatively Activated Macrophages. PLoS ONE 9(11): e112953. doi:10.1371/journal.pone.0112953Species: MouseSample type: Conditioned Media
  3. Karra L., Haworth O., Priluck R., et al. Lipoxin B4 promotes the resolution of allergic inflammation in the upper and lower airways of mice. Mucosal Immunol. 2014 Dec 3. doi: 10.1038/mi.2014.116.Species: MouseSample type: Serum
  4. Guo H., Yun C., Hou G., Du J., Huang X., et al. Mangiferin Attenuates Th1/Th2 Cytokine Imbalance in an Ovalbumin-Induced Asthmatic Mouse Model. PLOS One. Published: June 23, 2014. DOI: 10.1371/journal.pone.0100394Species: MouseSample type: Serum
  5. Zingariello, Maria, et al. "Characterization of the TGF-?1 signaling abnormalities in the Gata1 low mouse model of myelofibrosis."?Blood?121.17 (2013): 3345-3363.Species: MouseSample type: Conditioned Media
  6. Arevalo-Martin A., Molina-Holgado E., Guaza C. A CB?/CB? receptor agonist, WIN 55,212-2, exerts its therapeutic effect in a viral autoimmune model of multiple sclerosis by restoring self-tolerance to myelin. Neuropharmacology. 2012 Sep;63(3):385-93. doi: 10.1016/j.neuropharm.2012.04.012. Species: MouseSample type: Tissue Lysate
  7. Robbins D., Li W., Humphrey K., Zhao Y. Redox Factor-1 Mediates Inflammatory Response during Tumor Promotion in Skin Epidermal JB6 Cells. Open Journal of Apoptosis, 2012, 1, 19-24. http://dx.doi.org/10.4236/ojapo.2012.13003Species: MouseSample type: Cell Lysate
  8. Vila` L, Roglans N, Baena M, Barroso E, Alegret M, et al. (2012) Metabolic Alterations and Increased Liver mTOR Expression Precede the Development of Autoimmune Disease in a Murine Model of Lupus Erythematosus. PLoS ONE 7(12): e51118. doi:10.1371/journal.pone.0051118Species: MouseSample type: Conditioned Media
  9. Kelley P., et al. Regressed lymphatic vessels develop during corneal repair. Lab Invest. 2011 Nov;91(11):1643-51. doi: 10.1038/labinvest.2011.121.Species: MouseSample type: Tissue Lysate
  10. Ojeda, Gloria, et al. "Complement regulatory protein Crry/p65 costimulation expands natural Treg cells with enhanced suppressive properties in proteoglycan?induced arthritis."?Arthritis & Rheumatism?63.6 (2011): 1562-1572.Species: MouseSample type: Conditioned Media
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Very easy! I recommend their scanning service!!!
NIH
I used quite a a lot of cytokine and chemokine array kit from Raybiotech.These product is very simple and reliable to measure the production of cytokine/chemokine from cell culture media.They also provide a free Scanning service, so no need to worry about slide scanner. Highly recommended.
National Institute of health
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