Acetyl eIF5A/eIF5A2 (K47) Polyclonal Antibody
- Catalog No.:YK0021
- Applications:WB,ELISA
- Reactivity:Human,Mouse,Rat
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name:
- EIF5A2
- Protein Name:
- Eukaryotic translation initiation factor 5A-2
- Human Gene Id:
- 56648
- Human Swiss Prot No:
- Q9GZV4
- Mouse Gene Id:
- 208691
- Mouse Swiss Prot No:
- Q8BGY2
- Immunogen:
- The antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47. AA range:11-60
- Specificity:
- Acetyl-eIF5A/eIF5A2 (K47) Polyclonal Antibody detects endogenous levels of eIF5A/eIF5A2 protein only when acetylated at K47.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Rabbit
- Dilution:
- Western Blot: 1/500 - 1/2000.ELISA: 1/20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -20°C/1 year
- Other Name:
- EIF5A2; Eukaryotic translation initiation factor 5A-2; eIF-5A-2; eIF-5A2; Eukaryotic initiation factor 5A isoform 2
- Background:
- function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,tissue specificity:Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.,
- Function:
- function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,tissue specificity:Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.,
- Subcellular Location:
- nuclear pore,endoplasmic reticulum membrane,cytosol,intracellular membrane-bounded organelle,
- Expression:
- Brain,Epithelium,Ovarian carcinoma,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western blot analysis of L929 lysis using antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of lysate from L929 cells, using eIF5A (Acetyl-Lys47) Antibody.