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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
17β-HSD4 Polyclonal Antibody
- Catalog No.：YT5386
- Applications：WB,IHC-p,IF(paraffin section),ELISA
- Reactivity：Human,Mouse,Rat
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name：
- HSD17B4
- Protein Name：
- Peroxisomal multifunctional enzyme type 2
- Human Gene Id：
- 3295
- Human Swiss Prot No：
- P51659
- Mouse Gene Id：
- 15488
- Mouse Swiss Prot No：
- P51660
- Rat Gene Id：
- 79244
- Rat Swiss Prot No：
- P97852
- Immunogen：
- The antiserum was produced against synthesized peptide derived from the N-terminal region of human HSD17B4. AA range:41-90
- Specificity：
- 17β-HSD4 Polyclonal Antibody detects endogenous levels of 17β-HSD4 protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300.ELISA: 1/20000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- HSD17B4; EDH17B4; Peroxisomal multifunctional enzyme type 2; MFE-2; 17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4; D-bifunctional protein; DBP; Multifunctional protein 2; MPF-2
- MolecularWeight(Da)：
- 79686
- Observed Band(KD)：
- 80
- Background：
- hydroxysteroid 17-beta dehydrogenase 4(HSD17B4) Homo sapiensThe protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014],
- Function：
- catalytic activity:(24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl-CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA + H(2)O.,catalytic activity:(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.,disease:Defects in HSD17B4 are a cause of D-bifunctional protein deficiency (DBPD) [MIM:261515]. DBPD is a disorder of peroxisomal fatty acid beta-oxidation.,function:Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.,pathway:Lipid metabolism; fatty acid beta-oxidation.,similarity:Belongs to the short-chain dehydrogenases/reductases (SDR) family.,similarity:Contains 1 SCP2 domain.,tissue specificity:Present in many tissues with highest concentrations in liver, heart, prostate and testis.,
- Subcellular Location：
- mitochondrion,peroxisome,peroxisomal membrane,peroxisomal matrix,membrane,intracellular membrane-bounded organelle,
- Expression：
- Brain,Liver,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of HuvEc, K562 cells using 17β-HSD4 Polyclonal Antibody.. Secondary antibody(catalog#：RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
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- Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
- Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
- Western blot analysis of lysate from K562, HUVEC cells, using HSD17B4 Antibody.
