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蚂蚁淘在线 / 品牌中心 / Immunoway / Immunoway/Calnexin (phospho Ser583) Polyclonal Antibody/50ug 100ug/YP0041
商品描述
Calnexin (phospho Ser583) Polyclonal Antibody
- Catalog No.：YP0041
- Applications：WB,IHC-p,IF/ICC,ELISA
- Reactivity：Human,Mouse,Rat
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name：
- CANX
- Protein Name：
- Calnexin
- Human Gene Id：
- 821
- Human Swiss Prot No：
- P27824
- Mouse Gene Id：
- 12330
- Mouse Swiss Prot No：
- P35564
- Rat Gene Id：
- 29144
- Rat Swiss Prot No：
- P35565
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human Calnexin around the phosphorylation site of Ser583. AA range:543-592
- Specificity：
- Phospho-Calnexin (S583) Polyclonal Antibody detects endogenous levels of Calnexin protein only when phosphorylated at S583.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- CANX; Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
- MolecularWeight(Da)：
- 67568
- Observed Band(KD)：
- 90
- Background：
- calnexin(CANX)Homo sapiensThis gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008],
- Function：
- function:Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.,online information:Calnexin entry,similarity:Belongs to the calreticulin family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
- Subcellular Location：
- endoplasmic reticulum,endoplasmic reticulum lumen,endoplasmic reticulum membrane,smooth endoplasmic reticulum,rough endoplasmic reticulum,ribosome,membrane,axon,extracellular matrix,dendrite cytoplasm,melanosome,neuronal cell body,de
- Expression：
- B-cell lymphoma,Epithelium,Fibroblast,Keratinocyte,Kidney,Liver,Lymph,Placenta,Platelet,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Phospho-Calnexin (S583) Polyclonal Antibody diluted at 1：2000
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Calnexin (Phospho-Ser583) Antibody
- Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30", using Calnexin (Phospho-Ser583) Antibody. The lane on the right is blocked with the phospho peptide.
