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4000-520-616 / 18915418616
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0512-67156496
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商品描述
AChRα10 Polyclonal Antibody
- Catalog No.：YT0081
- Applications：WB,ELISA
- Reactivity：Human,Mouse,Rat
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name：
- CHRNA10
- Protein Name：
- Neuronal acetylcholine receptor subunit alpha-10
- Human Gene Id：
- 57053
- Human Swiss Prot No：
- Q9GZZ6
- Rat Gene Id：
- 64574
- Rat Swiss Prot No：
- Q9JLB5
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human CHRNA10. AA range:394-443
- Specificity：
- AChRα10 Polyclonal Antibody detects endogenous levels of AChRα10 protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- CHRNA10; NACHRA10; Neuronal acetylcholine receptor subunit alpha-10; Nicotinic acetylcholine receptor subunit alpha-10; NACHR alpha-10
- MolecularWeight(Da)：
- 49705
- Observed Band(KD)：
- 50
- Background：
- function:Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.,miscellaneous:The hetero-oligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).,similarity:Belongs to the ligand-gated ionic channel (TC 1.A.9) family.,subunit:Forms hetero-oligomeric channels in conjunction with CHRNA9. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 hetero-oligomers.,tissue specificity:Expressed in inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes.,
- Function：
- function:Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.,miscellaneous:The hetero-oligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).,simi
- Subcellular Location：
- acetylcholine-gated channel complex,membrane,integral component of membrane,cell junction,axon,perikaryon,postsynaptic membrane,
- Expression：
- Inner ear,Skeletal muscle,Tonsil,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
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- June 19-2018
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- FLOW-CYTOMEYRT-PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using AChRα10 Polyclonal Antibody
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- Western Blot analysis of A549 cells using AChRα10 Polyclonal Antibody
- Western blot analysis of lysates from HeLa, 293, COLO, and A549 cells, using CHRNA10 Antibody. The lane on the right is blocked with the synthesized peptide.
