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商品描述
V-ATPase D Polyclonal Antibody
- Catalog No.:YT4860
- Applications:WB,ELISA
- Reactivity:Human,Mouse,Rat
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name:
- ATP6V1D
- Protein Name:
- V-type proton ATPase subunit D
- Human Gene Id:
- 51382
- Human Swiss Prot No:
- Q9Y5K8
- Mouse Gene Id:
- 73834
- Mouse Swiss Prot No:
- P57746
- Immunogen:
- Synthesized peptide derived from V-ATPase D . at AA range: 70-150
- Specificity:
- V-ATPase D Polyclonal Antibody detects endogenous levels of V-ATPase D protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Rabbit
- Dilution:
- Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -20°C/1 year
- Other Name:
- ATP6V1D; ATP6M; VATD; V-type proton ATPase subunit D; V-ATPase subunit D; V-ATPase 28 kDa accessory protein; Vacuolar proton pump subunit D
- MolecularWeight(Da):
- 28263
- Observed Band(KD):
- 28
- Background:
- ATPase H+ transporting V1 subunit D(ATP6V1D)Homo sapiensThis gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq, Jul 2008],
- Function:
- function:Subunit of the peripheral V1 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.,similarity:Belongs to the V-ATPase D subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c", c"" and d).,
- Subcellular Location:
- lysosomal membrane,centrosome,cytosol,cilium,membrane,proton-transporting V-type ATPase complex,extracellular exosome,
- Expression:
- Bone marrow,Brain,Heart,Pancreatic adenocarcinoma,Pituitary,Placent
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- June 19-2018
- FLOW CYTOMEYRT PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western blot analysis of ATP6V1D Antibody. The lane on the right is blocked with the ATP6V1D peptide.