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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Chk2 (phospho-Ser19) rabbit pAb
- Catalog No.：YP1300
- Applications：WB
- Reactivity：Human
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name：
- CHEK2 CDS1 CHK2 RAD53
- Protein Name：
- Chk2 (Ser19)
- Human Gene Id：
- 11200
- Human Swiss Prot No：
- O96017
- Mouse Gene Id：
- 50883
- Mouse Swiss Prot No：
- Q9Z265
- Immunogen：
- Synthesized phosho peptide around human Chk2 (Ser19)
- Specificity：
- This antibody detects endogenous levels ofHuman Chk2 (phospho-Ser19)
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- WB 1:1000-2000
- Purification：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- Serine/threonine-protein kinase Chk2 (EC 2.7.11.1) (CHK2 checkpoint homolog) (Cds1 homolog) (Hucds1) (hCds1) (Checkpoint kinase 2)
- Observed Band(KD)：
- 61
- Background：
- checkpoint kinase 2(CHEK2)Homo sapiensIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutati
- Function：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,disease:Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.,disease:Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].,disease:Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807].,enzyme regulation:Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation.,function:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on "Ser-216", preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53
- Subcellular Location：
- chromosome, telomeric region,nucleoplasm,Golgi apparatus,PML body,
- Expression：
- Colon carcinoma,Mammary gland,Muscle,T-cell,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- June 19-2018
- FLOW CYTOMEYRT PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
