CatalogNo.: K-5900 Description: TheK-5900kithasbeendiscontinued.PleasecontactEcheloninindividualkitreagentsareneeded. Description:Echelon’sβ-catenin/Tcfprotein-proteininteractionassayisafluorescencepolarization(FP)assaydesignedforhighthroughputscreeningina384-wellformatforsmallmoleculeinhibitionortheβ-catenin:Tcfprotein-proteininteraction. | |||
β-cateninisakeymediatorfortheWntpathway.TheWntpathwayisclassifiedasaβ-catenin-dependentpathwayorβ-catenin-independentpathway.TheWnt/β-catenin-dependentpathwayisresponsIBLeformanycellularregulationssuchasproliferation,differentiationandcell-cellcommunication.HyperactivatedWnt/β-catenin-dependentpathwaytriggersprogressionofmanycancerssuchascolorectalcancers,livercancer,breastcancer,etc.ThetranscriptionoftheoveractiveWnt/β-catenin-dependentpathwayissolelydependentonthenuclearβ-catenin:T-cellfactor(Tcf)binding.Thus,inhibitorstargetingtheβ-catenin/Tcfprotein-proteininteractionappeartobeapromisingtherapeuticstrategyforcancer.Lately,literaturesuggestsactivationoftheβ-catenin/Tcfsignalingbythelipidgrowthfactor,lysophosphatidicacid(LPA),maybeinvolvedinneurogenesisaswell. StorageUponreceipt,storekitat-20°C.Allowthereagentstowarmtoroomtemperature.Centrifugevialsbeforeopening.References1)Lepourcelet,M,Chen,YN,etal.SmallmoleculeantagoNISTsoftheoncogenicTcf/beta-cateninproteincomplex.CancerCell.5(1),91-102(2004).2)Zhang,M,Huang,Z,etal.Newhomogeneoushigh-throughputassaysforinhibitorsof?-catenin/Tcfprotein–proteininteractions.AnalBiochem.424(1),57-63(2012).> |