Sphingo Strips are membranes pre-spotted with 15 different sphingolipids and blank control. Sphingo Strips are used in a simple protein-lipid overlay assay to help determine the specificity of your protein towards one or more of these lipids.
Briefly, once the membrane is blocked, it is incubated with your protein of interest. The membrane is then washed and incubated with an HRP labeled antibody against the protein or your proteins affinity tag. The membrane is washed again before incubation with ECL or a precipitating TMB detection reagent. This can then be visualized with the appropriate equipment based on the detection reagent used.
Sphingo Strips are 2 x 6 cm hydrophobic membranes with 100 pmol of the following lipids: Sphingosine, Sphingosine 1-phosphate (S1P), Phytosphingosine, Ceramide, Sphingomyelin, Sphingosylphosphorylcholine (SPC), Lysophosphatidic Acid (LPA), Myriosine, Monosialoganglioside-GM1, Disialoganglioside-GD3, Sulfatide, Psychosine, Cholesterol, Lysophosphatidylcholine (LPC), Phosphatidylcholine (PC).
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Echelon Biosciences成立于1997年10月3日,总部坐落于在美国犹他州。从1997到2004,EBI主要用于政府的补助金以及产品的销售收入来资助其研究工作。2005年初,EBI的依特钠Zentaris购买 (NAS:AEZS)的MEP抗感染,PI3激酶小分子程序,目录业务,与科学专业。2007年末,前沿科学公司(FSI)在洛根,犹他州购买了EBI, 专业从事卟啉和硼化学。自公司成立以来以来,EBI保留了开发研究试剂脂质新的检测方法。不断开拓**,从而推动药物研究的新发型。
Echelon Biosciences是美国杰出免疫学产品供应商,专业提供各种抗体、分析检测试剂盒、抑制剂等产品,尤其擅长以细胞信号、脂类代谢和标记物研究为主的相关产品。为研究治疗诸如癌症、糖尿病、炎症、感染和心脑血管疾病专家提供**有效工具。
Echelon Biosciences产品线
Lipid Kinases & Phosphatases
脂质激酶和磷酸
Lipid-Protein Interaction Tools
脂蛋白质相互作用工具
Lipid-Specific Assay Kits
脂质特定检测试剂盒
Lipid-Specific Antibodies & Binding Proteins
脂质特定抗体和结合蛋白
Hyaluronic Acid & Collagen IV Assay Kits
透明质酸和IV型胶原检测试剂盒
Synthetic Inositol phosphates, Lipids, & Phospholipids
合成的磷酸肌醇,脂和磷脂
PI3-Kinase PI3-激酶
Echelon Biosciences可提供PI3-Kinase 实验解决全套方案,从分析服务到实验用抗体、酶和抑制剂等,**满足科研需求。
专业系列
产品编号
特色产品
Kits
试剂盒
K-1000
PI3-K ELISA Kit
K-1000S
PI3-K ELISA: Pico Assay
K-1100
PI3-K Fluorescence Polarization Kit
K-1300
PI3-K AlphaScreen®
K-2700S
TRueFRET PI3-K Activity Assay
K-3000
Class III PI3-K ELISA Kit
Antibodies
抗体
Z-R015
Anti-huVps34 (Class III PI3-Kinase) Antibody for IP
Z-R016
Anti-huVps34 (Class III PI3-Kinase) Antibody for WB
Inhibitors
抑制剂
B-0021
Resveratrol (Class IA PI3-K Inhibitor)
B-0222
Wortmannin (PI3-Kinase Inhibitor)
B-0294
PI3-Kinase Inhibitor (LY294002)
B-0301
PI3-Kinase Gamma Inhibitor 1 (AS605240)
B-0302
PI3-Kinase Gamma Inhibitor 2 (AS604850
B-0303
PI3-Kinase Alpha Inhibitor 1 (PI-103)
B-0304
PI3-Kinase Alpha Inhibitor 2
Enzymes
酶
P27-18H
PI3-K (p-110 alpha/p85 alpha) Active Enzyme
P28-10H
PI3-K (p-110 beta/p85 alpha) Active Enzyme
Lysolipids 血溶性脂类
Lysolipid Pathway Synthetic Lysophosphatidic Acid (LPA)
◆ Inhibitors 抑制剂系列 ◆ Labeled 标记物系列
◆ Ligands 配体系列 ◆ Ligands 配体系列
◆ Analogs 类似物系列 ◆ Saturated 底物系列
◆ Unsaturated 不饱和系列
专业系列
产品标号
特色产品
Kits试剂盒
K-2800B
Lysophosphatidic Acid Competitive ELISA Kit
K-4100
Autotaxin Activity Assay
K-4200
Autotaxin Inhibitor Screening Kit
Enzymes酶
E-4000
Autotaxin Enzyme
Substrate底物
L-2000
FS-3 LysoPLD/Autotaxin Substrate
Inhibitors抑制剂
L-7416
LysoPLD/Autotaxin Inhibitor
Sphingolipids 鞘脂类
Synthetic Sphingolipids
◆ Sphingosine 鞘氨醇 ◆ Ceramide 神经酰胺
◆ Sphingosine 1 Phosphate (S1P ) 鞘氨醇磷酸酯 ◆ Sphingomyelin 鞘磷脂
◆ FTY720P
专业系列
产品编号
特色产品
Kits
试剂盒
K-1900
Sphingosine 1 Phosphate ELISA Kit (S1P)
K-3500
Sphingosine Kinase 1 Activity Assay
K-1800
Sphingomyelinase Assay Kit
K-3200
Acid Sphingomyelinase Assay Kit
Enzymes
酶
E-K068
Sphingosine Kinase 1, active
E-K069
Sphingosine Kinase 2, active
Inhibitors
抑制剂
B-0024
Sphingosine Kinase Inhibitor
产品编号
特色产品
S-400B
Sphingomyelin Biotin
P-B0SM
Sphingomyelin Beads
S-6100
Sphingosine Beads
S-6110
Sphingosine 1 Phosphate Beads
P-B00Ss
Sphingo Bead Sample Pack
G-SM01
Sphingomyelin Grip (Equinatoxin)
Autotaxin(ATX) 自分泌运动因子
Echelon Biosciences是世界上唯壹完整**提供Autotaxin(ATX) 和Autotaxin(ATX) 相关试剂的供应商。
产品编号
特色产品
K-4100
Autotaxin Activity Assay Kit
K-4200
Autotaxin Inhibitor Screening Kit
K-2800B
Lysophosphatidic Acid Competitive ELISA Kit
L-2000
FS-3 (LysoPLD / Autotaxin Substrate)
L-7416
BrP-LPA Autotaxin Inhibitor
E-4000
Autotaxin Enzyme