MutaGEL Oxidative Stress I PCR Assay
MutaGEL Oxidative Stress I PCR Assay manufactured in Germany by Immundiagnostik
Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only
Controls Included
Assay Principle
With the MutaGEL OxiStress I PCR Assay two DNA regions specific for the critical gene parts of eNOS and NAD(P)H Oxidase are amplified in parallel reactions. The amplification products are then treated with a mix of restriction enzymes, which are sensitive for the “negative” sequence variant of each gene – in parallel as well – such that in the following gel the genotype of the probe can be recognised for both polymorphisms from the length of the resulting restriction fragments (RFLP method).
Sample Preparation
- Extract total genomic DNA (for example from 200 µl of whole blood) using a commercially available DNA isolation kit.
- Start immediately with the amplification procedure or store the extracted DNA at < -18°C.
Products Related to MutaGEL OxiStress I PCR Assay
MutaGEL r-Vitamin D3 PCR Kit
MutaGEL r-Calcitonin PCR Assay Kit
MutaGEL Parodontitis PCR Assay
Restrictions
The PCR results for all positive controls in DNA fragments of indicated length and for samples at least in the amplification product indicated length. If this is not the case, the sample must be tested a second time or the complete analysis must be repeated with freshly isolated DNA. If there are no positive control DNA fragments present, the amplification was incorrect and the chosen PCR conditions have to been proven/ corrected.
EagleBio的5α-雄甾烷-3α，17β-二醇葡糖醛酸化物是C19类固醇，缩写为3α-DiolG，5α二醇G或简称为α二醇G。它主要作为睾丸激素和二氢睾丸激素（DHT）的代谢产物生产。它主要在目标外围组织（例如皮肤）中产生，尤其是在毛囊周围。大量的3α-DiolG刺激会导致过多的头发形成，特别是在女性通常不存在头发的地方。近年来，在研究患有特发性多毛症的妇女的临床研究人员中，人们对这种类固醇的测量越来越感兴趣。已知是3α-DiolG前体的类固醇包括脱氢表雄酮（DHEA），硫酸脱氢表雄酮（DHEAS），二氢睾丸酮（DHT），雄烯二酮和睾丸激素。已显示只有3α-DiolG随多毛症增加而随治疗减少。这种相关性在多囊卵巢综合征（PCO）患者中也得到了证明。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。
