iLite TNF-alpha Assay Ready Cells

TNF-alpha Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite® TNF-alpha Assay Ready Cells are intended for:

• Testing anti-TNF-alpha activity
• Testing NAb anti-TNF-alpha activity

Content: 1.5 ml of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), 10% glycerol, and 2.5% dimethyl sulfoxide (DMSO) .

Storage: -80°C, Cells should be used within 30 min of thawing.
For Research Use Only

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Key benefits of iLite® Assays

• Highly specific reporter gene cell lines
• Very sensitive cell line responses (>10 fold inductions)
• Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
• Assays within a workday (typically 4-7 hour assays)
• Normalization gene, which eliminates unwanted matrix effects

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Assay Principle

The iLite™ TNF-alpha Assay Ready Cells are engineered cells optimized to express Firefly luciferase under the control of a TNF-alpha responsive promoter and Renilla luciferase under the control of a constitutive promoter. TNF-alpha binds to the TNF receptor on the cell surface and activates the TNF-alpha regulated Firefly luciferase reporter gene construct. The Firefly luciferase signal can be measured in a luminometer following addition and incubation of the luciferase substrate. The Firefly luciferase signal is proportional to the functional activity of TNF-alpha in the sample. In the presence of TNF-alpha inhibitor activity in test samples, the amount of free TNF-alpha is reduced, resulting in a decreased stimulation of Firefly luciferase production. The Firefly luciferase signal is inversely proportional to the amount of anti-TNF-alpha activity in the samples. The constitutively expressed gene Renilla luciferase can be sequentially measured in the same wells as the Firefly luciferase, following addition and incubation of the Renilla substrate. This allows for normalization of results by compensating for cell number variation and eliminating potential matrix effects from a serum sample, for example. In samples with no suspected matrix effect, the Renilla reading may be excluded.

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Products Related to TNF-alpha Assay Ready Cells

TNF-Alpha ELISA Assay Kit
Human TNF-Alpha ELISA Assay
iLite ADCC anti-mTNF-alpha Activity Set

EagleBio的5α-雄甾烷-3α，17β-二醇葡糖醛酸化物是C19类固醇，缩写为3α-DiolG，5α二醇G或简称为α二醇G。它主要作为睾丸激素和二氢睾丸激素（DHT）的代谢产物生产。它主要在目标外围组织（例如皮肤）中产生，尤其是在毛囊周围。大量的3α-DiolG刺激会导致过多的头发形成，特别是在女性通常不存在头发的地方。近年来，在研究患有特发性多毛症的妇女的临床研究人员中，人们对这种类固醇的测量越来越感兴趣。已知是3α-DiolG前体的类固醇包括脱氢表雄酮（DHEA），硫酸脱氢表雄酮（DHEAS），二氢睾丸酮（DHT），雄烯二酮和睾丸激素。已显示只有3α-DiolG随多毛症增加而随治疗减少。这种相关性在多囊卵巢综合征（PCO）患者中也得到了证明。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。