Aflatoxin M1 Immunoaffinity Column

The Aflatoxin M1 Immunoaffinity Column is For Research Use Only

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Assay Instructions

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This Eagle Biosciences Aflatoxin M1 Immunoaffinity Column is for the clean-up of milk and milk products containing Aflatoxin M1. Immunoaffinity chromatography stands for an efficient enrichment process of aflatoxins with concomitant effective separation from matrix components. The eluate is best suited for determination by HPLC. By varying washing process, that means exchange PBS buffer with 0.2M ammonium acetate solution, GC can also be applied.

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Sample Preparation of Milk

According to the method of Shundo et al.1 the milk to be tested is first centrifuged by 1540g for 15 minutes. The cream is then separated using e.g. a separation funnel. If separation is incomplete, the milk sample should be filtered to avoid that remaining fatty particles block the column in next step below.

Cross Reactivity

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Aflatoxin M1 (AFM1): 100%
Aflatoxin B1 (AFB1): 98%
Aflatoxin B2 (AFB2): 99%
Aflatoxin G1 (AFG1): 50%
Aflatoxin G2 (AFG2): 98%

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Learn more about our Immunoaffinity Columns at Eagle Biosciences’ Product Highlights page dedicated to Immunoaffinity here:

Immunoaffinity Columns: Product Highlights

Product Developed and Manufactured in Germany by BioTeZ Berlin-Buch GmbH

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Related Products

Aflatoxin HPLC Immunoaffinity Column
Aflatoxin B1 ELISA Assay
Aflatoxin+Ochratoxin Immunoaffinity Column (3ml, 10 columns)

EagleBio的5α-雄甾烷-3α，17β-二醇葡糖醛酸化物是C19类固醇，缩写为3α-DiolG，5α二醇G或简称为α二醇G。它主要作为睾丸激素和二氢睾丸激素（DHT）的代谢产物生产。它主要在目标外围组织（例如皮肤）中产生，尤其是在毛囊周围。大量的3α-DiolG刺激会导致过多的头发形成，特别是在女性通常不存在头发的地方。近年来，在研究患有特发性多毛症的妇女的临床研究人员中，人们对这种类固醇的测量越来越感兴趣。已知是3α-DiolG前体的类固醇包括脱氢表雄酮（DHEA），硫酸脱氢表雄酮（DHEAS），二氢睾丸酮（DHT），雄烯二酮和睾丸激素。已显示只有3α-DiolG随多毛症增加而随治疗减少。这种相关性在多囊卵巢综合征（PCO）患者中也得到了证明。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。