Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit
Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit is Developed and Manufactured by Generic Assays
Size: 96 wells (24 samples x 4)
Incubation Time: 2 hours
Sample Type: Serum or Plasma
Number Of Samples Per Kit: 24 (22 samples + controls)
Sample Size: 50 µl
For Research Use Only
Controls Included
Assay Principle
Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit is a reagent kit for the determination of IgG antibodies against immunodominant major antigens of the SARS coronavirus-2. The test kit consists of modules separately coated with the major antigens of the virus:
The samples are pipetted horizontally over the 4 strips of a module (e.g. A1+A2+A3+A4), any antibodies present react with the specific antigens bound to the solid phase in the first reaction step. Unbound sample components are removed by a wash step after 45 minutes incubation at 37 °C.
The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 45 min at 37 °C, excessive conjugate is separated from the solid-phase immune complexes by the following wash step.
HRP converts the added colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature (18-25 °C) turning the solution from blue to yellow.
The optical density (OD) of the solution measured at 450 nm is directly proportional to the amount of specific antibodies bound.
Specimen collection and storage
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma samples (citrate, EDTA, heparin) can be used too, too. Hyperlipemic, hemolytic or contaminated samples must not be used and the samples must not contain preservatives.
Samples can be stored up to 5 days at 2 – 8 °C in the primary tubes. For longer storage, sera or plasmas extracted from the primary tubes must be frozen at -20°C. Repeated freezing and thawing should be avoided. If necessary, aliquots should be prepared before freezing.
Samples are diluted during the Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA test, controls should not be diluted.
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