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FAT10 Agarose is ideal for the enrichment of known FAT10-interacting proteins as well as the discovery of novel FAT10-interacting proteins. We recommend equilibrating the resin by washing with 5-10 mL of your desired aqueous buffer.

Source

E. coli-derived human FAT10 protein

Accession #

O15205/Q96EC7 (NP_006389)

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-920

Formulation	0.5 ml resin supplied in a 1 mL total volume of 50 mM HEPES pH 7.5, 250 mM NaCl, 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Do not freeze. 3 months from date of receipt, 2 to 8 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

Reconstitution Calculator

Background: FAT10

Human Leukocyte Antigen-F Associated Transcript 10 (FAT10), also known as Ubiquitin D (UBD), is a 165 amino acid (aa) member of the Ubiquitin-like family of proteins. Human FAT10 has a predicted molecular weight of 18.5 kDa and shares 69% aa sequence identity with mouse FAT10 (1). Human FAT10 mRNA is expressed as a single transcript in lymphoblastoid lines and dendritic cells, but more than one mRNA transcript has been identified for murine FAT10 (1,2). FAT10 can also be induced by IFN-gammaand TNF-alphain some cell lines (1). Structurally, FAT10 consists of two Ubiquitin-like domains that are connected by a short linker. Like Ubiquitin, FAT10 has a C-terminal glycine residue that can be used to form isopeptide bonds with target proteins. FAT10-conjugated proteins are targeted to the proteasome where the 26S Proteasome subunit S5a/Angiocidin binds to FAT10 and enables subsequent degradation of the conjugated protein (3). In addition to S5a/Angiocidin, FAT10 has been shown to interact with Huntingtin, Ataxin-1, MAD2, and NUB1L (4,5). FAT10 has been implicated in a number of biological processes such as cell cycle control, antigen presentation, and cytokine response (1,6-8). This protein iscovalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. It is useful for isolation and capture of FAT10 interacting proteins and/or enzymes that have an affinity for this Ubiquitin-like protein.

References