Recombinant Human HA-Ubiquitin Vinyl Sulfone Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
U-212
| Formulation | Supplied atX mg/ml (X μM) in 50 mM MES, pH 6.0 |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Ubiquitin
Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).
This N-terminal HA-tagged Ubiquitin is a potent, irreversible and specific inhibitor of most deubiquitinating enzymes (DUBs) including Ubiquitin C-terminal hydrolases (UCHs) and Ubiquitin-specific proteases (USPs). Useful for inhibiting the hydrolysis of poly-Ubiquitin chains on substrate proteins in vitro and thus enhances poly-Ubiquitin chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza Hemagglutinin protein. This epitope allows for the sensitive identification or purification of DUBs since it is specifically recognized be by Anti-HA antibodies and/or Anti-HA-agarose.
- Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
- Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
- Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
- Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
- Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
- Wei, W. et al. (2004) Nature 428:194.
- Wertz, I.E. et al. (2004) Nature 430:694.
Citations for Recombinant Human HA-Ubiquitin Vinyl Sulfone Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- Tumor necrosis factor related apoptosis inducing ligand (TRAIL) regulates deubiquitinase USP5 in tumor cellsAuthors: H Potu, M Kandarpa, LF Peterson, NJ Donato, M TalpazOncotarget, 2019;10(56):5745-5754.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymesAuthors: DS Hewings, J Heideker, TP Ma, AP AhYoung, F El Oualid, A Amore, GT Costakes, D Kirchhofer, B Brasher, T Pillow, N Popovych, T Maurer, C Schwerdtfe, WF Forrest, K Yu, J Flygare, M Bogyo, IE WertzNat Commun, 2018;9(1):1162.Species: HumanSample Types: ProteinApplications: Bioassay
- ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityAuthors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N MailandMol. Cell, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Immunoprecipitation
- A new gold(I) complex-Au(PPh3)PT is a deubiquitinase inhibitor and inhibits tumor growthAuthors: X Li, Q Huang, H Long, P Zhang, H Su, J LiuEBioMedicine, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Western Blot
- Inhibiting Multiple Deubiquitinases to Reduce Androgen Receptor Expression in Prostate Cancer CellsAuthors: A de Las Poz, T Reiner, V De Cesare, M Trost, C Perez-StabSci Rep, 2018;8(1):13146.Species: HumanSample Types: Cell LysatesApplications: Western Blot
- Usp9x regulates Ets-1 ubiquitination and stability to control NRAS expression and tumorigenicity in melanomaAuthors: H Potu, LF Peterson, M Kandarpa, A Pal, H Sun, A Durham, PW Harms, PC Hollenhors, U Eskiocak, M Talpaz, NJ DonatoNat Commun, 2017;8(0):14449.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Usp9x Promotes Survival in Human Pancreatic Cancer and Its Inhibition Suppresses Pancreatic Ductal Adenocarcinoma In Vivo Tumor GrowthAuthors: A Pal, M Dziubinski, MP Di Maglian, DM Simeone, S Owens, D Thomas, L Peterson, H Potu, M Talpaz, NJ DonatoNeoplasia, 2017;20(2):152-164.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Characterization of the deubiquitination activity and substrate specificity of the chicken ubiquitin-specific protease 1/USP associated factor 1 complexAuthors: H Zheng, M Wang, C Zhao, S Wu, P Yu, Y Lü, T Wang, Y AiPLoS ONE, 2017;12(11):e0186535.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Coinhibition of the deubiquitinating enzymes, USP14 and UCHL5, with VLX1570 is lethal to ibrutinib- or bortezomib-resistant Waldenstrom macroglobulinemia tumor cellsBlood Cancer J, 2016;6(11):e492.Species: HumanSample Types: ProteinApplications: Bioassay
- Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKC? Kinase ActivityAuthors: E Naik, VM DixitJ Immunol, 2016;196(8):3438-51.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Pyk2 Controls Integrin-Dependent CTL Migration through Regulation of De-AdhesionJ Immunol, 2016;0(0):.Species: MouseSample Types: Whole CellsApplications: Enzyme Assay
- PCNA-Dependent Cleavage and Degradation of SDE2 Regulates Response to Replication StressPLoS Genet, 2016;12(12):e1006465.Species: HumanSample Types: Whole CellsApplications: Enzyme Assay
- The proteasome deubiquitinase inhibitor VLX1570 shows selectivity for ubiquitin-specific protease-14 and induces apoptosis of multiple myeloma cellsSci Rep, 2016;6(0):26979.Species: HumanSample Types: ProteinApplications: Bioassay
- Ubiquitination of ROR?t at Lysine 446 Limits Th17 Differentiation by Controlling Coactivator RecruitmentJ Immunol, 2016;0(0):.
- USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphomaAuthors: Katharina EngelEMBO Mol Med, 2016;0(0):.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Differential role of the proteasome in the early and late phases of BDNF-induced facilitation of LTP.Authors: Santos A, Mele M, Vaz S, Kellermayer B, Grimaldi M, Colino-Oliveira M, Rombo D, Comprido D, Sebastiao A, Duarte CJ Neurosci, 2015;35(8):3319-29.Species: RatSample Types: Whole CellsApplications: Bioassay
- Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegumentprotein deneddylase to the nucleus of productively infected cells.Authors: Gastaldello S, Chen X, Callegari S, Masucci MPLoS Pathog, 2013;9(10):e1003664.Species: HumanSample Types: Whole CellsApplications: Bioassay
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