Recombinant Human His6-FLAG-p53 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
SP-452
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 450 mM NaCl, 10% Glycerol (v/v), 30μM ZnCl2 |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: p53
p53 is well known for its key role as a tumor suppressor protein.It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis includingcell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.
Citations for Recombinant Human His6-FLAG-p53 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transferAuthors: K Bhuripanyo, Y Wang, X Liu, L Zhou, R Liu, D Duong, B Zhao, Y Bi, H Zhou, G Chen, NT Seyfried, WJ Chazin, H Kiyokawa, J YinSci Adv, 2018;4(1):e1701393.Applications: Bioassay
- Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transferAuthors: K Bhuripanyo, Y Wang, X Liu, L Zhou, R Liu, D Duong, B Zhao, Y Bi, H Zhou, G Chen, NT Seyfried, WJ Chazin, H Kiyokawa, J YinSci Adv, 2018;4(1):e1701393.Applications: Bioassay
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