Lysozyme Conjugation Substrate, Biotin Summary
Lysozyme has been used extensively as a substrate to study the Ubiquitin-dependent proteolytic pathway in vitro. This protein is an N-end rule substrate, since the recognition of protein requires the binding of an E3 ligase to the N-terminal lysine residue. Ubiquitin-lysozyme conjugates can be formed in the presence of ATP, Ubiquitin and the appropriate conjugation enzyme source(s). This substrate protein can be used as a positive control with crude fractions (F-340, F-360, F-370, F-372) and conjugation kits (K-915, K-930,K-935, K-960). Additionally, this lysozyme is labeled with biotin for use with avidin affinity/detection reagents.
Specifications
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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