Recombinant Human SKP1/FBXO7/CUL1/RBX1 Complex Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-526
| Formulation | X mg/ml (XμM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: SKP1/FBX07/CUL1/RBX1 Complex
SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as FBXO7) that serve as the substrate recognition components of SCF ligases. FBXO7 (aka FBX7 or PARK15) plays a role in mitophagy by helping target Parkin (PARK2) to depolarized mitochondria, thereby inducing the ubiquitination of TOMM20and other mitochondrial proteins. Evidence from a Drosophila Parkin-/- neurodegeneration model indicates that FBXO7 can substitute for Parkin-mediated ubiquitination of mitochondrial substrates in some settings. This recombinant protein complex consists of His-tag SKP1, FLAG-tag FBXO7, His-tag CUL1 and untagged RBX1.
- Burchell V.S., et al. (2013) Nat. Neurosci. 16: 1257
- Chang Y.F. et al. (2006) Biochem. Biophys. Res. Comm. 342: 1022
- Hsu J.M., et al. (2004) J. Biol. Chem. 279: 32592
- Randle S.J. & Laman H. (2017) Curr. Protein Pept. Sci. 18: 715
- Stott S.R.W. et al. (2019) J. Pathol. 249: 241
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