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Reaction Conditions will be optimized for each specific application. In a 50 μl reaction containing 1 μM activated Parkin E3 ligase (E3-160 or E3-162), 1 μM His6-Miro1 is fully converted to Ubiquitinated forms in 60 minutes.

Source

E. coli-derived human Miro1/Rhot1 proteinContains an N-terminal 6-His tag, Ile186 - Thr591

Accession #

Q8IXI2

Predicted Molecular Mass

50 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-490

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: Miro1/Rhot1

Mitochondrial Rho GTPase 1 (also known as Miro1 or Rhot1) is a membrane associated GTPase with a predicted molecular weight of 71 kDa. Miro1 has been reported to directly associate with the KIF5 motor protein, allowing mitochondria to move along microtubules. A number of publications have identifed Miro1 as an in vivo substrate for the E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene), an essential component of the cellular machinery that participates in the removal of damaged mitochondria. This protein is useful as an in vitro substrate for activated recombinant Parkin enzyme.

References

Birsa, N. et al. (2014) J. Biol. Chem. 289: 14569
Kazlauskaite, A. et al. (2014) Open Biol. 4: 130213
Klosowiak, J.L. et al. (2016) Sci. Rep. 6: 33019
MacAskill, A.F. et al.(2009) Neuron 61: 541
Ordureau, A. et al. (2015) Proc. Natl. Acad. Sci. 112: 637

Long Name

Mitochondrial Rho (MIRO) GTPase 1

Entrez Gene IDs

55288 (Human)

Alternate Names

ARHT1; EC 3.6.5; EC 3.6.5.-; FLJ11040; FLJ12633; hMiro-1; Miro1; MIRO-1mitochondrial Rho GTPase 1; mitochondrial Rho 1; rac-GTP binding protein-like protein; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1; ras homolog gene family, member T1; Rhot1