Recombinant Viral HPV E6 Protein, CF Summary
Typical concentration to support E6AP-mediated p53 ubiquitination in vitro is 0.1-2 μM depending on experimental conditions.
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
AP-120
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.0, 400 mM NaCl, 2 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: E6
E6 (Early protein 6) is a viral protein produced in cells infected with the Human Papillomavirus. E6 forms a complex with the host cell ubiquitin ligase E6AP (E6-Associated-Protein) generating a ligase activity that polyubiquitinates tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. As a result DNA damage and chromosomal instabilities increase, often leading to cell proliferation and cancer. The E6/E6AP complex also targets other substrates for ubiquitination, such as TERT, BAK1, FADD, and pro-CASP8—none of which appear to be substrates for E6AP in the absence of E6. This protein is untagged, and contains six cysteine-to-serine substitutions at positions 23, 58, 87, 104, 118, and 147 (HPV type 16 sequence numbering).
- Beaudenon S. & Huibregtse J.M. (2008) BMC Biochem. 9 (Supp1): S4
- Zanier K., et al. (2007) Prot. Exp. & Purif. 51: 59
- Zanier K., et al. (2010) J. Mol. Biol. 396: 90
Citation for Recombinant Viral HPV E6 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transferAuthors: Y Wang, X Liu, L Zhou, D Duong, K Bhuripanyo, B Zhao, H Zhou, R Liu, Y Bi, H Kiyokawa, J YinNat Commun, 2017;8(1):2232.Species: YeastSample Types: Recombinant ProteinApplications: Bioassay
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