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Recombinant Human His6-UBE2J2-1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2J2-1 concentration of 0.1-1 μM.

Source

E. coli-derived human UBE2J2 proteinContains an N-terminal 6-His tag

Accession #

Q8N2K1

Predicted Molecular Mass

26 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-710

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UBE2J2

Ubiquitin-conjugating Enzyme E2, J2 (UBE2J2) is a 259 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 29 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs. UBE2J2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). This enzyme has been reported to be involved in the ER-associated degradation pathway (2). UBE2J2 has also been shown to interact with the mK3 Ubiquitin ligase (E3) and promote the ubiquitination of hydroxylated residues on major histocompatibility complex class I heavy chains (3). This protein contains an N-terminal 6-His tag.

References

Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
Kostova, Z. et al. (2007) Semin. Cell Dev. Biol. 18:770.
Wang, X. et al. (2009) J. Cell Biol. 187:655.

Long Name

Ubiquitin-conjugating Enzyme E2, J2

Entrez Gene IDs

118424 (Human); 140499 (Mouse); 298689 (Rat)

Alternate Names

EC 6.3.2.19; J2; NCUBE2; PRO2121; Ubc6; Ubc6p; UBE2J2; ubiquitin-conjugating enzyme E2 J2; ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast); yeast UBC6 homolog