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Recombinant Human His6-UBE2T is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2T concentration of 0.1-1 μM.

Source

E. coli-derived human UBE2T proteinContains an N-terminal 6-His tag

Accession #

NP_006348.1

Predicted Molecular Mass

24 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-695

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UBE2T

Ubiquitin-conjugating Enzyme E2T (UBE2T), also known as HSPC150 Protein Similar to Ubiquitin-conjugating Enzyme, is a member of the Ubiquitin-conjugating (E2) enzyme family. UBE2T has a predicted molecular weight of 22.5 kDa. The human protein shares 83% and 80% amino acid sequence identity with the mouse and rat orthologs, respectively. UBE2T has an E2 catalytic core domain with an active site cysteine residue and undergoes auto-ubiquitination on Lys91 and either Lys182 or Lys191 (1,2). UBE2T localizes to the nucleus and nucleolus and plays an important role in the Fanconi anemia DNA repair pathway (1,3). More specifically, UBE2T interacts with the RING domain of FANCL to mediate the ubiquitination of FANCD2 on Lys563 following exposure to mitomycin C (2,4,5). UBE2T has also been linked to the nucleotide excision repair pathway in chicken DT40 cells (6). UBE2T expression is downregulated under hypoxic conditions, and it may play a role in the development and/or progression of breast cancer (3,7). Thisprotein has an N-terminal His₆-tag.

References

Machida, Y.J. et al. (2006) Mol. Cell 23:589.
Alpi, A.F. et al. (2008) Mol. Cell 32:767.
Ueki, T. et al. (2009) Cancer Res. 69:8752.
Hodson, C. et al. (2011) J. Biol. Chem. 286:32628.
Sato, K. et al. (2012) Nucleic Acids Res. 40:4553.
Kelsall, I.R. et al. (2012) PLoS One 7:e36970.
Ramaekers, C.H. et al. (2011) Radiother. Oncol. 101:190.
Alpi A.F., et al. (2007) Mol. Cell Biol. 27: 8421.
Hao J., et al. (2008) Tumour Biol. 29: 195.
Longerich S., et al. (2009) J. Biol. Chem. 284: 23182.
Martinex-Forero I., et al. (2009) Clin. Canc. Res. 15: 6751.
Zhang J., et al. (2007) Cell. Cycle 7: 407.
Zhang J., et al. (2007) J. Genet. Genomics. 34: 573.

Long Name

Ubiquitin-conjugating Enzyme E2T

Entrez Gene IDs

29089 (Human); 67196 (Mouse); 360847 (Rat)

Alternate Names

Cell proliferation-inducing gene 50 protein; EC 6.3.2.19; HSPC150 protein similar to ubiquitin-conjugating enzyme; HSPC150; PIG50; UBE2T; Ubiquitin carrier protein T; ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme E2 T; ubiquitin-conjugating enzyme E2T (putative); Ubiquitin-protein ligase T