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Recombinant Human GST-UbcH2/UBE2H is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH2/UBE2H concentration of 0.1-1 μM.

Source

E. coli-derived human UbcH2/UBE2H proteinContains an N-terminal GST (glutathione S-transferase) tag

Accession #

P62256

Predicted Molecular Mass

46 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-605

Formulation	X mg/ml (XμM) in 50 mM HEPES, pH 8.0, 100 mM NaCl, and 10% Glycerol (v/v)
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UbcH2/UBE2H

Ubiquitin-conjugating Enzyme H2 (UbcH2), also known as Ubiquitin-conjugating Enzyme E2H (UBE2H), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). UbcH2/UBE2H has a predicted molecular weight of 20.5 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. Human UbcH2/UBE2H is highly expressed in skeletal and cardiac muscle, shows significant expression levels in kidney and placenta, and is expressed at low levels in liver, brain, lung, and spleen (2). TNF-alpha induces UbcH2/UBE2H expression via NF-kappa B, and SCL/Tal1 upregulates the expression of UbcH2/UBE2H during erythroid differentiation (2,3). Pathologically, a single nucleotide polymorphism within UbcH2/UBE2H is associated with autism (4). Furthermore, UbcH2/UBE2H has been reported as a putative oncogene for liver tumorigenesis (5). This protein has an N-terminal GST-tag. (Accession # NP_003335)

References

Kaiser, P. et al. (1994) J. Biol. Chem. 269:8797.
Li, Y.P. et al. (2003) FASEB J. 17:1048.
Lausen, J. et al. (2010) J. Biol. Chem. 285:5338.
Vourc"h, P. et al. (2003) Psychiatr. Genet. 13:221.
Keng, V.W. et al. (2009) Nat. Biotechnol. 27:264.

Entrez Gene IDs

7328 (Human); 22214 (Mouse)

Alternate Names

E2-14K; E2-20K; EC 6.3.2.19; UBC8; UBC8E2-20K; UBCH; UbcH2; UBE2H; Ubiquitin carrier protein H; ubiquitin-conjugating enzyme E2 H; Ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8); ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); Ubiquitin-protein ligase H