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Recombinant Human USP48 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP48 concentration of 200-500 nM.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP48 protein

Accession #

Q86UV5

Predicted Molecular Mass

120 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-614

Formulation	X mg/ml (X μM) in 50 mM HEPES pH7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP48

USP48 is a deubiquitinating enzyme (DUB) of the C19 peptidase family. Human USP48 has a predicted molecular weight of 119 kDa and is 93% and 94% identical to mouse and rat orthologues, respectively. USP48 has been reported to trim K48-linked poly-Ubiquitin chains, an activity that is regulated by Casein Kinase 2 (CK2)-mediated phosphorylation of the DUB. In conjunction with the COP9 signalosome (CSN), nuclear localized USP48 controls the proteasome-dependent turnover of activated NF-kappa B/RelA in the nucleus, thereby contributing to the control of immune responses. USP48 has also been indicated in the regulation of blood pressure and sodium balance via interactions with the dopamine D3 receptor (D3R) and the Na⁺-H⁺ exchanger. Finally, USP48 has been reported to stabilize Mdm2 in a manner that is not dependent on it deubiquitinating activity. This recombinant protein contains an N-terminal 6-His tag.

References

Armando I., et al. (2014) FASEB J. 28: 1422
Cetkovska K., et al. (2017) Sci.Rep. 7: 43180
Schweitzer K. & Naumann M.(2015) Biochim Biophys Acta. 1853: 453

Long Name

Ubiquitin Specific Protease 48

Entrez Gene IDs

84196 (Human); 170707 (Mouse); 362636 (Rat)

Alternate Names

Deubiquitinating enzyme 48; DKFZp762M1713; EC 3.1.2.15; EC 3.4.19.12; FLJ11328; FLJ20103; FLJ23054; FLJ23277; MGC132556; MGC14879; RAP1GA1; synUSP; ubiquitin carboxyl-terminal hydrolase 48; ubiquitin specific peptidase 48; ubiquitin specific protease 31; ubiquitin specific protease 48; ubiquitin thioesterase 48; Ubiquitin thiolesterase 48; Ubiquitin-specific-processing protease 48; USP31; USP48