Boston Biochem蚂蚁淘在线

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant humanMYSM1 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human MYSM1 protein

Accession #

Q5VVJ2

Predicted Molecular Mass

95 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-598

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: MYSM1

Histone H2A deubiquitinase MYSM1 is a specialized metalloprotease with a predicted molecular weight of 95 kDa. MYSM1 is a member of the peptidase M67A family and the human protein shares 79% amino acid sequence identity with its mouse ortholog. MYSM1 has been reported to function within a large chromatin remodeling complex, containing itself, PCAF, RBM10, TRIP5, and possibly other proteins. Biologically speaking, MYSM1 plays roles in hematopoiesis and lymphocyte differentiation, stem cell maintenance, and innate immunity.

References

Belle J.I., et al. (2016) Cell Death Differ. 2016 doi: 10.1038/cdd.2015.140.
Le Guen T., et al. (2015) J Allergy Clin. Immunol. 136: 1619
Panda S, Nilsson J.A., Gekara N.O. (2015) Immunity 43: 647
Zhu P., et al. (2007) Mol. Cell 27: 609

Long Name

Myb-Like, SWIRM And MPN Domains 1

Entrez Gene IDs

114803 (Human); 320713 (Mouse); 298247 (Rat)

Alternate Names

2A-DUB; DKFZp779J1554; DKFZp779J1721,2A-DUB; Histone H2A Deubiquitinase MYSM1; KIAA1915; Myb-like, SWIRM and MPN domains 1,2ADUB; MYSM1; RP4-592A1.1; SWIRM and MPN domain-containing protein 1