Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-568
| Formulation | X mg/ml total protein including USP1 (X µM) and UAF1(X µM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: USP1/UAF1 Complex
USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. Recombinant USP1 protein contains a C-terminal 6-His tag, and glycine-to-alanine substitutions at positions 670 and 671 (UniProt O94782 numbering). Recombinant UAF1 protein also contains a C-terminal 6-His tag. The assembled complex contains 25 µg each of USP1 and UAF1, resulting in a 1:1.2 stoichiometry.
- Cohn M.A., et al. (2009) J Biol. Chem. 284: 5343-5351
- Huang T.T., et al. (2006) Nat. Cell Biol. 8: 339-347
- Joo H-Y., et al. (2011) Biol. Chem. 286: 7190-7201
- Moretti J., et al. (2012) J Biol. Chem. 287: 29429-29441
- Williams S.A, et al. (2011) Cell 146: 918–930
Citations for Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
4Citations: Showing 1 - 4Filter your results:
Filter by:
- Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometryAuthors: AP Lawson, DW Bak, DA Shannon, MJC Long, T Vijaykumar, R Yu, FE Oualid, E Weerapana, L HedstromOncotarget, 2017;8(31):51296-51316.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometryAuthors: AP Lawson, DW Bak, DA Shannon, MJC Long, T Vijaykumar, R Yu, FE Oualid, E Weerapana, L HedstromOncotarget, 2017;8(31):51296-51316.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia PathwayAuthors: Sylvie van TwestMol. Cell, 2016;0(0):.Applications: Bioassay
- USP7 is a SUMO deubiquitinase essential for DNA replicationAuthors: E Lecona, S Rodriguez-, J Specks, AJ Lopez-Cont, I Ruppen, M Murga, J Muñoz, J Mendez, O Fernandez-Nat Struct Mol Biol, 2016;23(4):270-7.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF
Average Rating: 5(Based on 1 Review)
Have you used Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
