Recombinant Human His6-USP9x Isoform 2 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-552
| Formulation | X mg/ml (X μM) in 50 mMHEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 5 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: USP9x
Ubiquitin SpecificPeptidase 9, X-linked (USP9x), also known as DFFRX, FAF, and FAM, is acytoplasmic protein that belongs to the peptidase C19 family ofdeubiquitinating enzymes (1,2). It is 2570 amino acids (aa) in length with apredicted molecular weight of 292.3 kDa. Human USP9x shares 98% aa sequenceidentity with the mouse and rat orthologs. USP9x contains a catalytic domainlocated between aa 1531 and 1971 and a Ubiquitin-like domain that is located ataa 873-963 (3). It is widely expressed and has been shown to interact with anddeubiquitinate multiple substrates. Some putative targets includebeta -Catenin, TOR, Synuclein-alpha, the Ubiquitin ligases (E3s) Itch, MARCH7, and SMURF1, and the MAP kinase kinase kinase ASK1 (4-10). USP9x has also been shown to control the activity of the AMPK-related kinases ARK5 and MARK4 through direct removal of Lys29- and Lys33-linked Ubiquitin chains (3). It has also been shown to promote cell survival by deubiquitinating and stabilizing the anti-apoptotic protein Mcl-1, and to regulate chromosome alignment and segregation during mitosis by deubiquitinating the chromosome passenger protein Survivin (11-13). Additionally, USP9x appears to promote TGF-betasignaling via deubiquitination of mono-ubiquitinated Smad4 (14). USP9x expression has been shown to be correlated with several cancer types (11,15,16). This recombinant human protein contains an N-terminal His6-tag.
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Citations for Recombinant Human His6-USP9x Isoform 2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- The deubiquitinase USP9X regulates FBW7 stability and suppresses colorectal cancerAuthors: OM Khan, J Carvalho, B Spencer-De, R Mitter, D Frith, AP Snijders, SA Wood, A BehrensJ. Clin. Invest., 2018;0(0):.Applications: Bioassay
- Grb2-Mediated Recruitment of USP9X to LAT Enhances Themis Stability following Thymic SelectionAuthors: A Garreau, G Blaize, J Argenty, N Rouquié, A Tourdès, SA Wood, A Saoudi, R LesourneJ. Immunol., 2017;0(0):.Species: HumanSample Types: ProteinApplications: Bioassay
- Grb2-Mediated Recruitment of USP9X to LAT Enhances Themis Stability following Thymic SelectionAuthors: A Garreau, G Blaize, J Argenty, N Rouquié, A Tourdès, SA Wood, A Saoudi, R LesourneJ. Immunol., 2017;0(0):.Species: HumanSample Types: ProteinApplications: Bioassay
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