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苏州工业园区生物纳米园A4#216
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0512-67156496
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商品描述
Biological Activity Technical DataAdditional InformationBackground Product DatasheetsCalculators
Biological Activity
Prototypical PI 3-kinase inhibitor (IC50 values are 0.31, 0.73, 1.06 and 6.60 μM for PI 3-Kβ, PI 3-Kα, PI 3-Kδ and PI 3-Kγ respectively). Also inhibits other kinases including, CK2, mTOR, PLK1, PIM1 and PIM3. Inhibits proliferation and induces apoptosis in human colon cancer cells in vitro and in vivo. Suppresses proliferation of mESCs. Inhibits autophagic sequestration in rat hepatocytes.
Negative Control also available.
Technical Data
M.Wt:
343.81
Formula:
C19H17NO3.HCl
Solubility:
Soluble to 25 mM in DMSO and to 5 mM in ethanol
Purity:
≥99%
Storage:
Store at RT
CAS No:
934389-88-5
The technical data provided above is for guidance only.For batch specific data refer to the Certificate of Analysis.Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Other Product-Specific Information:
- Negative Control Available
- View R&D Systems' research posterfeaturing LY 294002 hydrochloride
Background References
- The selectivity of protein kinase inhibitors: a further update.Bain et al.Biochem.J., 2007;408:297
- Phosphoinositide 3-kinase inhibitor LY294002 but not serum withdrawal suppresses proliferation of murine embryonic stem cells.Lianguzova et al.Cell Biol.Int., 2007;31:330
- The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes.Blommaart et al.Eur.J.Biochem., 1997;243:240
- A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY 294002).Vlahos et al.J.Biol.Chem., 1994;269:5241
Product Datasheets
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Citations for LY 294002 hydrochloride
The citations listed below are publications that use Tocris products.Selected citations for LY 294002 hydrochloride include:
115Citations: Showing 1 - 10
- Extracorporeal shock waves protect cardiomyocytes from DOX-induced cardiomyopathy by upregulating survivin via the integrin-ILK-Akt-Sp1/p53 axis.Authors: Lee Et al.Sci Rep2019;9:12149
- The standardized Korean Red Ginseng extract and its ingredient ginsenoside Rg3 inhibit manifestation of breast cancer stem cell-like properties through modulation of self-renewal signaling.Authors: Oh Et al.J Ginseng Res2019;43:421
- Dual RXR motifs regulate nerve growth factor-mediated intracellular retention of the delta opioid receptor.Authors: Shiwarski Et al.Mol Biol Cell2019;30:680
- Human vascular cell responses to the circulating bone hormone osteocalcin.Authors: Millar Et al.J Cell Physiol2019;234:21039
- The Protective Effect of Ins. on Rat Cortical Neurons in Oxidative Stress and Its Dependence on the Modulation of Akt, GSK-3beta, ERK1/2, and AMPK Activities.Authors: Zakharova Et al.Int J Mol Sci2019;20
- Characterization of the brain penetrant neuropeptide Y Y2 receptor antagonist SF-11.Authors: Domin Et al.ACS Chem Neurosci2019;10:3454
- Intracellular emetic signaling evoked by the L-type Ca2+ channel agonist FPL64176 in the least shrew (Cryptotis parva).Authors: Zhong Et al.Eur J Pharmacol2018;834:157
- The homozygous CX3CR1-M280 mutation impairs human monocyte survival.Authors: Collar Et al.JCI Insight2018;3
- PI3Kδ activates E2F1 synthesis in response to mRNA translation stress.Authors: Gnanasundram Et al.Nat Commun2017;8:2103
- A pluripotent stem cell-based model for post-implantation human amniotic sac development.Authors: ShaoNat Commun2017;8(1):208
- Structural and pharmacological basis for the induction of mitochondrial biogenesis by formo. but not clenbu.Authors: CameronSci Rep2017;7(1):10578
- CFTR-mediated anion secretion across intestinal epithelium-like Caco-2 monolayer under PTH stimulation is dependent on intermediate conductance K+ channels.Authors: Jantarajit Et al.Am J Physiol Cell Physiol2017;313:C118
- PI3K class II α regulates δ-opioid receptor export from the trans-Golgi network.Authors: Shiwarski Et al.Mol Biol Cell2017;28:2202
- Phosphoinositide-dependent enrichment of actin monomers in dendritic spines regulates synapse development and plasticity.Authors: Lei Et al.J Cell Biol2017;216:2551
- Signaling Cascade Involved in Rapid Stimulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by dexameth.Authors: Bossmann Et al.Int J Mol Sci2017;18
- Isolation and characterization of a new naturally immortalized human breast carcinoma cell line, KAIMRC1.Authors: Ali Et al.BMC Cancer2017;17:803
- Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress.Authors: Oh Et al.Mol Med Rep2017;15:2583
- Adenosine A3 receptor elicits chemoresistance mediated by multiple resistance-associated protein-1 in human glioblastoma stem-like cells.Authors: Torres Et al.Oncotarget2016;7:67373
- miR-382 targeting PTEN-Akt axis promotes liver regeneration.Authors: Bei Et al.Elife2016;7:1584
- The GABAB positive allosteric modulators CGP7930 and GS39783 stimulate ERK1/2 signalling in cellslacking functional GABAB receptorsAuthors: Olianas Et al.European Journal of Pharmacology2016;794:135
- Tumor suppression in basal keratinocytes via dual non-cell-autonomous functions of a Na,K-ATPase beta subunit.Authors: Hatzold Et al.Elife2016;5
- Cannabinoid receptor agonist WIN55,212-2 and fatty acid amide hydrolase inhibitor URB597 may protect against cognitive impairment in rats of chronic cerebral hypoperfusion via PI3K/AKT signalingAuthors: Shao-Hua Su Et al.Behavioural Brain Research2016;313:334
- Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal CellsAuthors: Mou Et al.Cell: Stem Cell2016;19:217
- Pyrrolidine dithiocarbamate activates the Nrf2 pathway in astrocytes.Authors: Liddell Et al.J Neuroinflammation2016;13:49
- Poly(I:C) increases the expression of mPGES-1 and COX-2 in rat primary microglia.Authors: Oliveira Et al.Int J Mol Med2016;13:11
- Progesterone receptor membrane component-1 regulates hepcidin biosynthesis.Authors: Li Et al.J Clin Invest2016;126:389
- Up-Regulated Expression of LAMP2 and Autophagy Activity during Neuroendocrine Differentiation of Prostate Cancer LNCaP CellsAuthors: Morell Et al.PLoS One2016;11:e0162977
- Phosphoinositide-3-Kinase Is the Primary Mediator of Phosphoinositide-Dependent Inhibition in Mammalian Olfactory Receptor Neurons.Authors: Ukhanov Et al.Front Cell Neurosci2016;10:97
- Drug-based modulation of endogenous stem cells promotes functional remyelination in vivo.Authors: Najm Et al.Int J Cancer2015;522:216
- Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron.Authors: Lindström Et al.J Biomed Sci2015;3:e04000
- The thymoquinone-induced production of reactive oxygen species promotes dedifferentiation through the ERK pathway and inflammation through the p38 and PI3K pathways in rabbit articular chondrocytes.Authors: Yu and KimAlcohol Clin Exp Res2015;35:325
- Acetate supplementation as a means of inducing glioblastoma stem-like cell growth arrest.Authors: Long Et al.J Cell Physiol2015;230:1929
- Dimeric dipeptide mimetics of the nerve growth factor Loop 4 and Loop 1 activate TRKA with different patterns of intracellular signal transduction.Authors: Gudasheva Et al.Nature2015;22:106
- PI3K Phosphorylation Is Linked to Improved Electrical Excitability in an In Vitro Engineered Heart Tissue Disease Model System.Authors: Kana Et al.Tissue Eng Part A2015;21:2379
- Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic neurons.Authors: Ledonne Et al.Mol Psychiatry2015;20:959
- A novel mechanism for cytoprotection against hypoxic injury: δ-opioid receptor-mediated increase in Nrf2 translocation.Authors: Cao Et al.Oncotarget2015;172:1869
- SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II.Authors: Choi Et al.PLoS One2015;136:E219
- An in vitro model of latency and reactivation of varicella zoster virus in human stem cell-derived neurons.Authors: Markus Et al.PLoS Pathog2015;11:e1004885
- Upregulation of Nicotinic Acetylcholine Receptor α4+β2 through a Ligand-Independent PI3Kβ Mechanism That Is Enhanced by TNFα and the Jak2/p38Mapk Pathways.Authors: Rogers and GahringJ Neuroinflammation2015;10:e0143319
- Glucocorticoids Distinctively Modulate the CFTR Channel with Possible Implications in Lung Development and Transition into Extrauterine Life.Authors: Laube Et al.PLoS One2015;10:e0124833
- Palladin mediates stiffness-induced fibroblast activation in the tumor microenvironment.Authors: McLane and LigonBiophys J2015;109:249
- ER-α36-mediated rapid estrogen signaling positively regulates ER-positive breast cancer stem/progenitor cells.Authors: Deng Et al.BMC Cancer2014;9:e88034
- Angiogenic growth factors augment K-Cl cotransporter expression in erythroid cells via hypoxia-inducible factor-1α.Authors: Gonsalves Et al.Am J Hematol2014;89:273
- Non-cell-autonomous mechanism of activity-dependent neurotransmitter switching.Authors: Guemez-Gamboa Et al.Neuron2014;82:1004
- Interaction of mTOR and Erk1/2 signaling to regulate oligodendrocyte differentiation.Authors: Dai Et al.Glia2014;62:2096
- Rapid elevation of sodium transport through Ins is mediated by AKT in alveolar cells.Authors: Mattes Et al.Physiol Rep2014;2:e00269
- Decrease of PKB/Akt Phosphorylation is Partially Mediated by SAPK/JNK Activation in Serum-free L6 Myoblasts Starved with Low Glucose.Authors: Kim Et al.J Phys Ther Sci2014;26:1757
- δ-Opioid receptors up-regulate excitatory amino acid transporters in mouse astrocytes.Authors: Liang Et al.J Exp Bot2014;171:5417
- BDNF-endocannabinoid interactions at neocortical inhibitory synapses require phospholipase C signaling.Authors: ZhaoJ Neurophysiol2014;111:1008
- Characterisation of AmphiAmR11, an amphioxus (Branchiostoma floridae) D2-DA-like G protein-coupled receptor.Authors: Bayliss and EvansPLoS One2013;8:e80833
- Acetate supplementation induces growth arrest of NG2/PDGFRα-positive oligodendroglioma-derived tumor-initiating cells.Authors: Long Et al.PLoS One2013;8:e80714
- Curcumin suppresses migration and proliferation of Hep3B hepatocarcinoma cells through inhibition of the Wnt signaling pathway.Authors: Kim Et al.Mol Med Rep2013;8:282
- ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change.Authors: Montané and MenandNeuroscience2013;64:4361
- PI3K-Akt signaling activates mTOR-mediated epileptogenesis in organotypic hippocampal culture model of post-traumatic epilepsy.Authors: Berdichevsky Et al.J Neurosci2013;33:9056
- Melatonin acts through MT1/MT2 receptors to activate hypothalamic Akt and suppress hepatic gluconeogenesis in rats.Authors: Faria Et al.Am J Physiol Endocrinol Metab2013;305:E230
- Cellular Ins resistance disrupts leptin-mediated control of neuronal signaling and transcription.Authors: Nazarians-Armavil Et al.Mol Endocrinol2013;27:990
- Label-free integrative pharmacology on-target of opioid ligands at the opioid receptor family.Authors: Morse Et al.BMC Pharmacol Toxicol2013;14:17
- Activation of AMPK inhibits cervical cancer cell growth through AKT/FOXO3a/FOXM1 signaling cascade.Authors: Yung Et al.J Steroid Biochem Mol Biol2013;13:327
- PI 3-kinase independent role for AKT in F-actin regulation during outer segment phagocytosis by RPE cells.Authors: Bulloj Et al.Exp Eye Res2013;113:9
- Melatonin induces transcriptional regulation of Bim by FoxO3a in HepG2 cells.Authors: Carbajo-Pescador Et al.Br J Cancer2013;108:442
- Ceramide 1-phosphate stimulates proliferation of C2C12 myoblasts.Authors: Gangoiti Et al.Biochimie2012;94:597
- Fucoidan from seaweed Fucus vesiculosus inhibits migration and invasion of human lung cancer cell via PI3K-Akt-mTOR pathways.Authors: Lee Et al.PLoS One2012;7:e50624
- PPARδ activation protects endothelial function in diabetic mice.Authors: Tian Et al.Diabetes2012;61:3285
- PI3K/Akt signaling pathway in the basolateral amygdala mediates the rapid antidepressant-like effects of trefoil factor 3.Authors: Shi Et al.Neuropsychopharmacology2012;37:2671
- Nucleus accumbens mGluR5-associated signaling regulates binge alcohol drinking under drinking-in-the-dark procedures.Authors: Cozzoli Et al.Cancer Res2012;36:1623
- Cervical spinal erythropoietin induces phrenic motor facilitation via extracellular signal-regulated protein kinase and Akt signaling.Authors: Dale Et al.J Neurosci2012;32:5973
- Curcumin inhibits HCV replication by induction of heme oxygenase-1 and suppression of AKT.Authors: Chen Et al.Int J Mol Med2012;30:1021
- A screen for regulators of survival of motor neuron protein levels.Authors: Makhortova Et al.Nat Chem Biol2011;7:544
- Ligand-directed functional selectivity at the mu opioid receptor revealed by label-free integrative pharmacology on-target.Authors: Morse Et al.PLoS One2011;6:e25643
- Astragaloside IV stimulates angiogenesis and increases hypoxia-inducible factor-1α accumulation via phosphatidylinositol 3-kinase/Akt pathway.Authors: Zhang Et al.J Pharmacol Exp Ther2011;338:485
- The canonical nuclear factor-κB pathway regulates cell survival in a developmental model of spinal cord motoneurons.Authors: Mincheva Et al.J Neurosci2011;31:6493
- JNK suppression is essential for 17β-OE inhibits prostaglandin E2-Induced uPA and MMP-9 expressions and cell migration in human LoVo colon cancer cells.Authors: Hsu Et al.J Med Food2011;18:61
- A positive cross-regulation of HER2 and ER-α36 controls ALDH1 positive breast cancer cells.Authors: Kang Et al.J Pharmacol Exp Ther2011;127:262
- Neurotoxicity induced by okadaic acid in the human neuroblastoma SH-SY5Y line can be differentially prevented by α7 and β2* nicotinic stimulation.Authors: Barrio Et al.Toxicol Sci2011;123:193
- TRPV4-mediated calcium influx into human bronchial epithelia upon exposure to diesel exhaust particles.Authors: Li Et al.Environ Health Perspect2011;119:784
- Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.Authors: Yip Et al.PLoS Biol2010;8:e1000399
- Extranuclear estrogen receptors mediate the neuroprotective effects of estrogen in the rat hippocampus.Authors: Yang Et al.PLoS One2010;5:e9851
- Pharmacological and genetic accumulation of hypoxia-inducible factor-1alpha enhances excitatory synaptic transmission in hippocampal neurons through the production of vascular endothelial growth factor.Authors: Huang Et al.J Neurosci2010;30:6080
- Leptin regulates AMPA receptor trafficking via PTEN inhibition.Authors: Moult Et al.J Neurosci2010;30:4088
- Mechano-transduction in osteoblastic cells involves strain-regulated estrogen receptor alpha-mediated control of Ins-like growth factor (IGF) I receptor sensitivity to Ambient IGF, leading to phosphatidylinositol 3-kinase/AKT-dependent Wnt/LRP5 receptor-iAuthors: Sunters Et al.J Biol Chem2010;285:8743
- miR-155 and its star-form partner miR-155* cooperatively regulate type I IF. production by human plasmacytoid dendritic cells.Authors: Zhou Et al.Blood2010;116:5885
- Level of G protein-coupled receptor kinase-2 determines myocardial ischemia/reperfusion injury via pro- and anti-apoptotic mechanisms.Authors: Brinks Et al.J Cell Sci2010;107:1140
- Optimal experimental design for parameter estimation of a cell signaling model.Authors: Bandara Et al.PLoS Comput Biol2009;5:e1000558
- FcεRI-induced activation by low antigen concentrations results in nuclear signals in the absence of degranulation.Authors: Grodzki Et al.Circ Res2009;46:2539
- Activation of glycogen synthase kinase-3 β is required for hyperDA and D2 receptor-mediated inhibition of synaptic NMDA receptor function in the rat prefrontal cortex.Authors: Li Et al.J Biol Chem2009;29:15551
- Regulation of p53 expression, phosphorylation and subcellular localization by a G-protein-coupled receptor.Authors: Solyakov Et al.Oncogene2009;28:3619
- PLZF is a regulator of homeostatic and cytokine-induced myeloid development.Authors: Doulatov Et al.Genes Dev2009;23:2076
- Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis.Authors: Wang Et al.Mol Biol Cell2009;20:4153
- Ethanol augments RANTES/CCL5 expression in rat liver sinusoidal endothelial cells and human endothelial cells via activation of NF-kappa B, HIF-1 alpha, and AP-1.Authors: Yeligar Et al.J Immunol2009;183:5964
- Ethanol-induced expression of ET-1 and ET-BR in liver sinusoidal endothelial cells and human endothelial cells involves hypoxia-inducible factor-1alpha and microrNA-199.Authors: Yeligar Et al.J Immunol2009;183:5232
- c-jun controls the ability of IL-12 to induce IL-10 production from human memory CD4+ T cells.Authors: Garcia Et al.J Immunol2009;183:4475
- C5a mediates peripheral blood neutrophil dysfunction in critically ill patients.Authors: Morris Et al.Am J Respir Crit Care Med2009;180:19
- Soy phytoestrogens are neuroprotective against stroke-like injury in vitro.Authors: Schreihofer and RedmondJ Neurosci2009;158:602
- Efficacy of signal pathway inhibitors alone and in combination with cisp. varies between human non-small cell lung cancer lines.Authors: Kilic Et al.J.Surg.Res.2009;154:9
- Blockade of estrogen receptor signaling inhibits growth and migration of medulloblastoma.Authors: Belcher Et al.Endocrinology2009;150:1112
- Mechanisms underlying fibronectin-induced up-regulation of P2X4R expression in microglia: distinct roles of PI3K-Akt and MEK-ERK signalling pathways.Authors: Tsuda Et al.J Cell Mol Med2009;13:3251
- Inulin increases glucose transport in C2C12 myotubes and HepG2 cells via activation of AMP-activated protein kinase and phosphatidylinositol 3-kinase pathways.Authors: Yun Et al.PLoS One2009;12:1023
- Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells.Authors: Cho Et al.J Vet Sci2009;10:23
- PI3 kinase/Akt activation mediates estrogen and IGF-1 nigral DA neuronal neuroprotection against a unilateral rat model of Parkinson's disease.Authors: Quesada Et al.Dev Neurobiol2008;68:632
- Reactive oxygen species stabilize hypoxia-inducible factor-1 alpha protein and stimulate transcriptional activity via AMP-activated protein kinase in DU145 human prostate cancer cells.Authors: Jung Et al.Carcinogenesis2008;29:713
- Phosphatidylinositol 3-kinase is a key mediator of central sensitization in painful inflammatory conditions.Authors: Pezet Et al.J Neurosci2008;28:4261
- Temporal profiling of changes in phosphatidylinositol 4,5-bisphosphate, inositol 1,4,5-trisphosphate and diacylglycerol allows comprehensive analysis of phospholipase C-initiated signalling in single neurons.Authors: Nelson Et al.J Neurochem2008;107:602
- Hallucinogens recruit specific cortical 5-HT(2A) receptor-mediated signaling pathways to affect behavior.Authors: González-Maeso Et al.Exp Mol Med2007;53:439
- Resveratrol stimulates glucose transport in C2C12 myotubes by activating AMP-activated protein kinase.Authors: Park Et al.Br J Pharmacol2007;39:222
- cis-9,trans-11-conjugated linoleic acid down-regulates phorbol ester-induced NF-kappaB activation and subsequent COX-2 expression in hairless mouse skin by targeting IkappaB kinase and PI3K-Akt.Authors: Hwang Et al.Carcinogenesis2007;28:363
- Regulation of DA transporter function and cell surface expression by D3 DA receptors.Authors: Zapata Et al.J Biol Chem2007;282:35842
- IGF-I inhibition of apoptosis is associated with decreased expression of prostate apoptosis response-4.Authors: Chung Et al.J Endocrinol2007;194:77
- Ins induces airway smooth muscle contraction.Authors: Schaafsma Et al.J Biomed Sci2007;150:136
- PI-3-kinase-dependent membrane recruitment of centaurin-α2 is essential for its effect on ARF6-mediated actin cytoskeleton reorganisation.Authors: Venkateswarlu Et al.Neuron2007;120:792
- Cyclin D1 is necessary for tamoxifen-induced cell cycle progression in human breast cancer cells.Authors: Kilker and Planas-SilvaMol Immunol2006;66:11478
- Differential reelin-induced enhancement of NMDA and AMPA receptor activity in the adult hippocampus.Authors: Qiu Et al.Br J Pharmacol2006;26:12943
- Ischemic preconditioning protects by activating prosurvival kinases at reperfusion.Authors: Hausenloy Et al.Am J Physiol Heart Circ Physiol2005;288:H971
- Glucose deprivation increases mRNA stability of vascular endothelial growth factor through activation of AMP-activated protein kinase in DU145 prostate carcinoma.Authors: Yun Et al.J Neurosci2005;280:9963
- LY 294002 inhibits adenosine receptor activation by a mechanism independent of effects on PI-3 kinase or casein kinase II.Authors: Searl and SilinskyPurinergic Signal2005;1:389
- Phospholipase C-linked receptors regulate the ATP-sensitive potassium channel by means of phosphatidylinositol 4,5-bisphosphate metabolism.Authors: Xie Et al.Proc Natl Acad Sci U S A1999;96:15292
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Reviews for LY 294002 hydrochloride
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PI3K inhibition decreased the LPA induced expression of pAMPK.
By lisha joshion 06/18/2019
Application: Species:Mouse
BV2 microglia cells were incubated for one hour with 10µM LY294002 prior to treatment with 1µM LPA. Western blot analysis revealed a possible role for PI3K in the LPA induced expression of pAMPK.
LY 294002 blocks LPA-mediated COX-2 expression
By Anonymouson 12/13/2018
Application: Species:Human
Human osteosarcoma cells were incubated with 10 μM LY 294002 for 30 min prior to treatment with 10 μM LPA to estimate COX-2 expression using Western blot analysis. LY 294002 abolished LPA-induced COX-2 expression.
LY 294002 inhibits 15d-PGJ2-induced Akt phosphorylation
By Christopher Trummeron 10/15/2018
Application: Species:Human
MG-63 cells were incubated with PD169316 (25 μM), LY294002 (10 μM) or Akt-1/2 (5 μM Akt-I) for 30 min prior to 15d-PGJ2 treatment (20 μM) for 1 h to follow pAkt expression using Western blot. Akt-1/2 completely blocked 15d-PGJ2-induced Akt phosphorylation in MG-63 cells.
Successfull PI 3-kinase inhibitor
By Anonymouson 01/09/2018
Application: Species:Human
I used LY294002 hydrochloride 10µM to inhibit pAKT response of FFAR GPCR activators (15min 37°C).
Reduction of Foxo1 phosphorylation in cultured theca cells by LY294002.
By Zhenmin Leion 01/04/2018
Application: Species:Mouse
Mouse ovarian theca cells isolated from 60-day old mice were used. Cultured Ptenfl/fl and tPtenMT ovarian theca cells were pretreated with vehicle (DMSO), LY294002 (50 mM), or API-2 (7.5 mM) for 1 h before the addition of LH (50 ng/mL) for 30 min. Western blotting was used to detect Foxo1 phosphorylation in cultured ovarian theca cells.
