- 10X Restriction Enzyme Reaction Buffer 3
- 基因表达载体和病毒
- Prigrow CI Medium
- 1kb Plus Opti-DNA Marker
- 10X Restriction Enzyme Reaction Buffer 2
- 抗体
- PriCoat Flasks
- 细胞生物学
- Prigrow VI Medium
- 2019冠状病毒疾病(Coronavirus Disease 2019);
- 10X Universal Restriction Enzyme Reaction Buffer
- Prigrow X Series Medium for T5403
- 1kb Opti DNA标记
- miRNA一体化cDNA合成试剂盒
- 100bp Opti-DNA Marker
- 普锐斯系列T0768培养基
- 高保真™ 保真度DNA聚合酶
- PriCoat T75烧瓶,过滤器盖
- 10X Restriction Enzyme Reaction Buffer 1
- Prigrow XII培养基
- 分子生物学和PCR
Description | PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb – 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size. Total DNA concentration for all bands combined is 103.2ng/μl. |
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SKU | G248 |
Applications | For approximating DNA size and amount on agarose gels. |
Bands | 14 |
DNA Range | 250bp-25kb |
Note | Concentration of this marker is 104 μg / ml |
Suggested Load | Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel. |
Shipping Conditions | Shipped on blue ice packs. |
Storage Condition | Store @ 4°C for up to 3 months or -20°C for up to 24 months. |
Unit quantity | 500 μl/100 loads |
- DNA Marker Guide
- 1kb Plus Opti-DNA Marker
Is it ready for use? | |
Yes, it is ready to use. Just load suggested volume (5ul) to the well. |
What is the smallest size agarose gel can separate? | |
That is around 300bp for 1% agarose and 100bp for 2.5% agarose. With anything smaller than the dye, there may be separation but it will be difficult to tell when the fragment will run off the gel. |
Are the Opti-DNA markers compatible with radiolabelling? | |
Our lab has not tested the Opti-DNA markers for this particular application, but we believe it shouldn"t be a problem. The Opti-DNA markers should be compatible for radio-labelling. |