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商品描述
| Product Name | Collagen (Type I), FITC conjugated *Water Insoluble* |
| Size | 5 mg |
| Catalog # | AS-85102 |
| US$ | $104 |
Besides water-soluble collagen (Type I) conjugate (Cat#85111), AnaSpec also offers water-insoluble fluoresceinated collagen (type I). It can also be used for fluorometric measurement of collagenase activity. Cat#85102 is the water-insolulbe collagen that is heavily labeled with FITC, resulting in almost total quenching of the conjugates fluorescence. Protease-catalyzed hydrolysis slowly yields brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. Although this fluoresceinated collagen is more slowly digested by MMP-1 than water-soluble collagen (Cat#85111), it is more specific for the enzyme. Minimum Purity: 95% | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| References | Sela MN, et al. (2003). Enzymatic degradation of collagen-guided tissue regeneration membranes by periodontal bacteria. Clin Oral Implants Res 14, 263-8, Baici A, et al. (1980). A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils. Anal Biochem 108, 230-2. |
| Molecular Weight | N/D |
| Spectral Properties | Abs/Em = 492/515 nm |
