| Product Name | Calpain Substrate, Fluorogenic(Z - AA)2Rh110 |
| Size | 5 mg |
| Catalog # | AS-60320-5 |
| US$ | $118 |
A very sensitive fluorogenic calpain substrate, Abs/Em=501/527 nm. In general, Rh110-based protease substrates are much more sensitive than the analogous AMC or AFC-based substrate. This enhanced sensitivity might be attributed to the greater fluorescence of the enzymatic product. The colorless and nonfluorescent (Z-Ala-Ala)2Rh110 is hydrolyzed to the highly fluorescent Rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Alternatively, (Z-Ala-Ala)2Rh110 can also be used to detect calpain in a chromogenic mode since the enzymatic product (Rhodamine 110) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). After cleavage, Abs(max) is at 497 nm and Em(max) is at 527 nm. | |
| Detailed Information |  Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| References | Gitler D and Spira ME (1998). Real time imaging of calcium-induced localized proteolytic activity after axotomy and its relation to growth cone formation. Neuron 20, 1123-35, Rothe G, et al. (1992).Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54, Rothe G, et al. (1992).Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54. |
| Molecular Weight | 885.6 |
| (Z-AA)2Rh110 | |
| Sequence(Three-Letter Code) | (Z - Ala - Ala)2Rh110 |
| Spectral Properties | Abs/Em = 497/520 nm |
| Solvent System | DMSO |
