| Product Name | Fluorescein - Trp25 - Exendin - 4 (FLEX)HGEGTFTSDLSKQMEEEAVRLFIE - (Trp - S - FAM) - LKNGGPSSGAPPPS - NH2 |
| Size | 0.5 mg |
| Catalog # | AS-63899 |
| US$ | $312 |
| Purity | % Peak Area By HPLC ≥ 95% |
This peptide is Exendin-4 labeled withfluorescein at the Tryptophan residue. FLEX is equipotent to GLP-1(7-36)-amide and exendin-4 as an inhibitor of [125I] GLP-1 binding to the human GLP-1 receptor stably expressed in CHO cells, and maintains full biological potency and efficacy as measured by the stimulation of cAMP accumulation in these cells. FLEX binding to CHO/hGLP-1R membranes results in an increase in fluorescence anisotropy. The binding is specific and saturable (Kd = 2.0 +/- 0.4 nM), and GLP-1(7-36)-amide and Exendin-4 are equipotent inhibitors of FLEX binding to the human GLP-1 receptor. Thus, FLEX is a potent, biologically active ligand that is useful for the study of the binding and functional characteristics of the human GLP-1 receptor. | |
| Detailed Information |  Material Safety Data Sheets (MSDS) |
| Storage | -20°C |
| References | Chicchi, GG. et al. Peptides 18, 319 (1997), doi: 10.1016/S0196-9781(96)00320-8 |
| Molecular Weight | 4606.4 |
| HGEGTFTSDLSKQMEEEAVRLFIE-(Trp-S-FAM)-LKNGGPSSGAPPPS-NH2 | |
| Sequence(Three-Letter Code) | H - His - Gly - Glu - Gly - Thr - Phe - Thr - Ser - Asp - Leu - Ser - Lys - Gln - Met - Glu - Glu - Glu - Ala - Val - Arg - Leu - Phe - Ile - Glu - (Trp - S - FAM) - Leu - Lys - Asn - Gly - Gly - Pro - Ser - Ser - Gly - Ala - Pro - Pro - Pro - Ser - NH2 |
| Product Citations | Rajan, S. et al. (2015). Chronic hyperglycemia downregulates GLP-1 receptor signaling in pancreatic β-cells via protein kinase A. Mol Metabolism 4, 265. doi: http://dx.doi.org/10.1016/j.molmet.2015.01.010. |
