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101Bio/101Bio HRP Substrate Kit/30 ml / 30 ml/P5W1

价格
面议
货号:P5W1
浏览量:127
品牌:101Bio
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商品描述
101Bio HRP Substrate Kit

Name

101Bio HRP Substrate Kit

Cat. #

P5W1
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Application

Western Blot

Ready to use, strong and long-lasting signal (>4h), low background.

This product is for research use only.

Product Size

30 ml / 30 ml

Description

101Bio HRP substrate kit provides high intensity signal and low background detection of low abundance endogenous proteins. This highly sensitive ECL substrate is available in a two-bottle format for Western blotting with horseradish peroxidase (HRP) enzyme conjugates.This high-performance substrate will pick up your most difficult-to-detect proteins in Western Blots.The ECL substrate is compatible with different membranes, blocking reagents and a wide range of antibody dilutions. 101Bio HPR substrate kit provides excellent performance, versatility and long-lasting signal for high quality data.

  • Ready-to-use
  • Strong - pick up your most difficult-to-detect proteins
  • long-lasting signal (>4h)
  • low background

Shipping / Storage

Ship at room temperature.Store at 4℃

Shelf Life

12 months

Manual (protocol)

101Bio.com HRP Substrate Kit

Components

ComponentsAmountStorage
Substrate A30 ml4℃
Substrate B30 ml4℃
Protein Analysis Reagents
NameCat. #Size
101Bio HRP Substrate KitP5W130 ml /30 ml
101Bio Antibody EnhancerP5W230 ml
101Bio WB Stripping SolutionP5W3250 ml
101Bio Ponceau S StainP5W4250 ml
101Bio Peroxidase SuppressorP5W530 ml
101Bio High Efficiency Protein Precipitation kitP5W630 ml /30 ml
101Bio Red Blood Cell Lysis BufferP5W7250 ml
101Bio WB Blocking SolutionP5W8250 ml
101Bio Denaturing Protein Solubilization ReagentP5W910 ml
101Bio Non-Denatured Protein Solubilization ReagentP5W1010 ml
101Bio Protein Solubilization Reagent for MSP5W112 ml
101Bio SDS-RemoverP5W1210 ml
101Bio Albumin Depletion Reagent for Plasma and SerumP5W1310 ml
101Bio Protein A+G Agarose BeadsW03491 ml
101Bio Protease Inhibitor Cocktail (100x)W22001 ml
101Bio Yeast Total Protein Extraction Kit for SDS-PAGEY203-5050 rxn
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101bio外泌体提取试剂盒在获得极低白蛋白含量的外泌体中的应用外泌体(Exosomes)是大多数正常细胞和病变细胞都能够分泌的一种细胞外囊泡,粒子直径大约在30-150nm之间。外泌体广泛存在于血浆、乳液、尿液、唾液等体液中,并可携带母细胞中的多种蛋白、脂类、DNA、RNA等物质,参与细胞通讯、细胞迁移、血管新生和肿瘤细胞生长等过程。外泌体最早在19世纪80年代就有记载,当时人们只把它当做一种细胞废弃物。2013年诺贝尔生理学或医学奖被授予James E. Rothman等科学家,以表彰他们发现囊泡转运的调控机制。近年来,由于外泌体的特殊性质,研究人员对外泌体的研究热情激增,并发现其在诊断与治疗领域存在巨大潜力。在外泌体研究中,外泌体的分离提取是一直困扰广大研究人员的一大难题。外泌体领域资深科学家Clotilde Théry所建立的超速离心提取法一直被奉为外泌体提取的“金标准”。但超速离心法由于需要样本量大,耗时长,依赖特定仪器,提取量低,获得外泌体纯度低等缺点,大大降低了研究人员的研究效率。随着外泌体研究的不断发展,一些商品化试剂盒应运而生,这些试剂盒大多采用PEG沉淀原理,只需在半个小时左右的时间就可完成提取。但由于提取过程中PEG的加入,使获得的外泌体中含有PEG偶联物,严重影响后续实验的进行。另外,无论是超速离心法还是PEG沉淀试剂盒,所分离的外泌体中都含有大量的白蛋白,这也给后续的实验造成很大的干扰。针对外泌体提取过程所遇见的问题,美国101bio公司研发并生产了一款基于密度梯度分离法的外泌体分离试剂盒。该试剂盒相对于密度梯度离心省去了超速离心的步骤,而且还利用了外泌体特定极性和所带电荷等特性,比传统的密度梯度离心要简单很多,产量也高很多。最主要的是,通过对比101bio外泌体提取试剂盒所提取的外泌体白蛋白含量远远低于超速离心法和PEG沉淀法试剂盒所提取的外泌体,这样为研究人员后续实验扫清了一大障碍。另外,通过粒径分析,101bio外泌体提取试剂盒所提取的微粒直径在30-150nm之间的高达96.3%,说明该试剂盒所提取的外泌体几乎不包含其他类型微粒。