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蚂蚁淘在线 / 品牌中心 / 101Bio / 101Bio/3D Tumor Culture Gel/10 mL/P720S-10

101Bio/3D Tumor Culture Gel/10 mL/P720S-10

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货号：P720S-10
浏览量：127
品牌：101Bio
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商品描述
Home »products » Cell Biology Product » 3D Tumor Culture Gel
3D Cell Culture Gel - Col-Tgelfor tumor cell, stem cell and primary cell
Col-Tgel Flyer
Cat.#StiffnessVolumeStrength3 Stiffness Application
P720S-2Soft2 mL0.9 ~ 1.5 kPaCirculating cells, Nerve cells, BT474, HL60, P39
P720S-10Soft10 mL
P720M-2Med2 mL14 ~ 20 kPaMuscle cells, Adipose cells, C2C12, HepG2
P720M-10Med10 mL
P720H-2Stiff2 mL35 ~ 47 kPaBone cells
P720H-10Stiff10 mL
P720SMH-6S/M/H2 mL x 3A combo of P720S-2, P720M-2, P720H-2 for customer testing their cells.
Customer can mix any two gels at any ratio to make their own special stiffness gel.
How to pay with
Also can buy from :
P720SMH-6, Fisher Cat.# NC1291700

Application

Col-Tgel is a tailorable 3D cell culture matrix for tumor cell, stem cell and primary cell. We provide soft, medium and stiff gel for your different cell types.

It provides collagen backbone for cell attachment and allows customizing matrix components by additional extracellular matrix, growth factors, cytokines, and/or hormones for optimizing three dimensional cell culture.

This product is for research use only.

Stiffness selection for different cell types

3D Cell Culture Gel - 3 stiffness

Click to see full size image.

Formation of tumor spheroids in Col-Tgel

Col-Tgel for tumor cell, stem cell and primary cell

Fang, JY., etc. PloS one 9, no. 8 (2014): e105616.

For immunohistochemistry and immunocytochemistry method, please refer to Page 2 of this publication.

Unique Features

  • Handle at room temperature (no need on ice)
  • Save money: 30% below market price
  • Save time: No hanging drop, rotating wall vessel bioreactors
  • 3 stiffness(unique feature): to fit most cell types
  • 2 mL aliquotes: avoid freeze-thaw cycle - user friendly
  • Cytokine / Hormon free: no background
  • Flexible:

    -Col-Tgel can be customized for different experiments.- Geometry: 3D embedding, 2D seeding on the top of hydrogel, or unique molds.
    -Sample size and quantity: 6- to 96- well with/without tissue culture insert or transwell. - Matrix component and stiffness: collagen-based with different yield strength (i.e. Soft, Med and Stiff).
    - Transparency: easy to observe directly under microscopes and can apply with immunohistochemistry directly.

    • Col-Tgel is a tailorable collagen-based, cell remodelable hydrogel system. It not only can be used a carrier for cell or drug delivery, but also in vitro cell culture platforms. Besides, it contains cell essential nutrients, and provides a stable and durable platform for long-term studies such as cell-cell, cell-matrix interactions, and etc.
3D Cell Culture Gel - Col-Tgel for tumor cell, stem cell and primary cell, easy handling

Shipping / Storage

Ship at room temperature.Store Component A (gel) at 4oC, and store Component B (crosslinker) at -80oC for 9 months shelf life, or at -20oC for 3 months, or at 4oC for 1 month.Avoid freeze-thaw cycle for Component B.

Shelf Life

Component A: 12 months at 4oC

Component B: 9 months at -80oC; 3 months at -20oC; 1 month at 4oC

Manual (protocol)

101Bio.com 3D Cell Culture Gel - Col-Tgel

Citations

Citation 1:
Kuwahara, Kenrick, Zhi Yang, Ginger C. Slack, Marcel E. Nimni and Bo Han.Cell delivery using an injectable and adhesive transglutaminase–gelatin gel.Tissue Engineering Part C: Methods 16, no. 4 (2009): 609-618.
Citation 2:
Kuwahara, Kenrick, Josephine Y. Fang, Zhi Yang and Bo Han.Enzymatic crosslinking and degradation of gelatin as a switch for bone morphogenetic protein-2 activity. Tissue Engineering Part A 17, no. 23-24 (2011): 2955-2964.
Citation 3:
Fang, Josephine, Zhi Yang, ShihJye Tan, Charisse Tayag, Marcel E. Nimni, Mark Urata and Bo Han.Injectable gel graft for bone defect repair.Regenerative medicine 9, no. 1 (2014): 41-51.
Citation 4:
Tan, ShihJye, Josephine Y. Fang, Zhi Yang, Marcel E. Nimni and Bo Han. The synergetic effect of hydrogel stiffness and growth factor on osteogenic differentiation.Biomaterials 35, no. 20 (2014): 5294-5306.
Citation 5:
Fang, Josephine Y., Shih-Jye Tan, Zhi Yang, Charisse Tayag and Bo Han. Tumor Bioengineering Using a Transglutaminase Crosslinked Hydrogel.PloS one 9, no. 8 (2014): e105616.
Citation 6:
Shih-Jye Tan, Fang, Josephine Y., Y. Wu, Zhi Yang, G. Liang and Bo Han.Muscle tissue engineering and regeneration through epigenetic reprogramming and scaffold manipulation.Sci Rep. 2015 Nov 9;5:16333. doi: 10.1038/srep16333.
Citation 7:
Lee G, Kim HJ, Kim HM.RhoA-JNK Regulates the E-Cadherin Junctions of Human Gingival Epithelial Cells.J Dent Res. 2015 Dec 3. pii: 0022034515619375. [Epub ahead of print]
Citation 8:
Fang JY, Tan SJ, Wu YC, Yang Z, Hoang BX, Han B.From competency to dormancy: a 3D model to study cancer cells and drug responsiveness.J Transl Med. 2016 Feb 4;14(1):38. doi: 10.1186/s12967-016-0798-8.
* Different names (TGase gel, TG gel) were used in old publications.They referred to the same 3D cell culture gel, and we finally name it as Col-Tgel.
See all 101Bio"s citation papers, click here
3D Cell Culture Gel - Col-Tgel for tumor cell, stem cell and primary cell, easy handling
3D Cell Culture Gel - Col-Tgel for tumor cell, stem cell and primary cell, in vitro and in vivo
Tumor and regular tissue progression and development in Col-Tgel for in vitro and in vivo application.
Exosome IsolationPurity > 95%
Protein Extraction1 min total protein, 40 min membrane protein
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Endotoxin-Free Plasmid Kitsmaxi, midi and mini-prep
101bio外泌体提取试剂盒在获得极低白蛋白含量的外泌体中的应用外泌体（Exosomes）是大多数正常细胞和病变细胞都能够分泌的一种细胞外囊泡，粒子直径大约在30-150nm之间。外泌体广泛存在于血浆、乳液、尿液、唾液等体液中，并可携带母细胞中的多种蛋白、脂类、DNA、RNA等物质，参与细胞通讯、细胞迁移、血管新生和肿瘤细胞生长等过程。外泌体最早在19世纪80年代就有记载，当时人们只把它当做一种细胞废弃物。2013年诺贝尔生理学或医学奖被授予James E. Rothman等科学家，以表彰他们发现囊泡转运的调控机制。近年来，由于外泌体的特殊性质，研究人员对外泌体的研究热情激增，并发现其在诊断与治疗领域存在巨大潜力。在外泌体研究中，外泌体的分离提取是一直困扰广大研究人员的一大难题。外泌体领域资深科学家Clotilde Théry所建立的超速离心提取法一直被奉为外泌体提取的“金标准”。但超速离心法由于需要样本量大，耗时长，依赖特定仪器，提取量低，获得外泌体纯度低等缺点，大大降低了研究人员的研究效率。随着外泌体研究的不断发展，一些商品化试剂盒应运而生，这些试剂盒大多采用PEG沉淀原理，只需在半个小时左右的时间就可完成提取。但由于提取过程中PEG的加入，使获得的外泌体中含有PEG偶联物，严重影响后续实验的进行。另外，无论是超速离心法还是PEG沉淀试剂盒，所分离的外泌体中都含有大量的白蛋白，这也给后续的实验造成很大的干扰。针对外泌体提取过程所遇见的问题，美国101bio公司研发并生产了一款基于密度梯度分离法的外泌体分离试剂盒。该试剂盒相对于密度梯度离心省去了超速离心的步骤，而且还利用了外泌体特定极性和所带电荷等特性，比传统的密度梯度离心要简单很多，产量也高很多。最主要的是，通过对比101bio外泌体提取试剂盒所提取的外泌体白蛋白含量远远低于超速离心法和PEG沉淀法试剂盒所提取的外泌体，这样为研究人员后续实验扫清了一大障碍。另外，通过粒径分析，101bio外泌体提取试剂盒所提取的微粒直径在30-150nm之间的高达96.3%，说明该试剂盒所提取的外泌体几乎不包含其他类型微粒。