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ECM BIOSCIENCES/CD55 (Extracellular region) M002 Antibody/100 μl/CM0021

价格
¥5900.00
货号:CM0021
浏览量:127
品牌:ECM BIOSCIENCES
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商品描述

CD55, also known as Decay-accelerating factor (DAF) is an inhibitor of the complement system, and is broadly expressed in malignant tumours. In cancer, CD55 has been implicated in tumorigenesis, neoangiogenesis, and metastasis. CD55 may decrease complement mediated tumor cell lysis, inhibit tumor apoptosis, and promote invasive cancer cell motility. These roles in cancer may involve binding to the seven-span transmembrane receptor CD97. In neuroblastoma cells, CD55 contributes to growth of colonies and to invasion of cells, but not to stemness. In neuroblastoma cells, CD55 is upregulated in a small population of cells that are HIF-2α positive. This CD55 positive subpopulation is highly invasive and has low adhesion to fibronectin and collagen. In addition, CD55 expression correlates with poor prognosis in neuroblastoma patients.

 

References

Cimmino F et al. (2016) Oncogenesis 5:e212.Mikesch JH et al.(2006) Cell Oncol. 28(5-6):223.

Western blot analysis of native lysates: human A549 (lane 1), MDA-MB-231 (lane 2), and A431 (lane 3) cell lines, and lung tissue (lane 4). The blot was probed with mouse monoclonal anti-CD55 (CM0021) at 1:500.

Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0021). The antibody was detected using goat anti-mouse DyLight® 594.

Clone M002 was generated from proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line.
*For more information, see UniProt Accession P08174
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M002 detects 60-80 kDa* bands corresponding to the molecular mass of CD55 on SDS-PAGE immunoblots of "native" A431, A549, and MDA-MB-231 cell lysates. The antibody also detects a "native" recombinant human CD55 extracellular region, but does not detect the denatured form of CD55. The antibody works for native western blot, immunoprecipitation, protein ELISA, and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY

ECM BIOSCIENCES的A431细胞裂解液A431细胞在细胞表面表达约106种表皮生长因子(EGF)受体。在用EGF刺激后,A431细胞的EGF受体磷酸化水平显着增加,随后激活了主要的细胞信号传导途径,例如PKB / Akt和MAP激酶途径。在EGF刺激后的不同时间点,这些细胞信号通路的下游,各种细胞骨架,细胞质和核蛋白被磷酸化。在含有10%胎牛血清的DMEM中生长的A431细胞的融合培养物在1%SDS,1.0 mM原钒酸钠,10 mM Tris(pH 7.4)缓冲液中裂解。在稀释至最终浓度和缓冲液之前,使用BCA方法(Pierce)确定蛋白质浓度。