Overview
Product name
Streptavidin Alexa Fluor® 647Monovalent Antibody Labeling KitProduct overview
StreptavidinAlexa Fluor®647 Monovalent Antibody Labeling Kit ab272190 is used to label biotinylated antibodies withAlexa Fluor®647.
The Antibody - StreptavidinAlexa Fluor®647 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.
Several antibodies can be labeled with this kit, and its associated kits, and used together in the same experiment.Use this kit to:- label biotinylated antibodies with fluorescent dyes, and use them in immunostaining just like a directly dye conjugated antibody
- label two or morebiotinylated antibodies with different fluorescent dyes* and then use them together in the same experiment*multiple kits required, see list below
- label as little as 0.1 µg of biotinylated antibodyin less than 5 mins without washes
Technical notesThe 50µg size of this kit is typically sufficient to label 50µg of antibody, the 25 µg size is sufficient to label25 µgof antibody.
When the antibody - streptavidin complex is produced with this kit, due to the monovalent nature of the streptavidin and the blocking solution, there is no risk of forming aggregates of multiple antibodies.
The antibody - streptavidin complex is also very stable, if 2 or more biotinylated antibodies are labeled with different dyes, and then mixed, no dye will transfer between the antibodies.
List of available monovalent streptavidin antibody labeling kits
Streptavidin Alexa Fluor®488 (ab272187)Streptavidin Atto 542 (ab272188)Streptavidin Alexa Fluor®594 (ab272189)Streptavidin Alexa Fluor®647 (ab272190)– this kit
Note: the streptavidin in this kit contains very small amounts of non-monovalent streptavidin, when used with the blocking buffer included in the kit, this does not affect the conjugated antibodies.
Notes
Alexa Fluor®is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA oroutlicensing@thermofisher.com.
Tested applications
Suitable for:Flow Cyt, IHCmore details
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 50 µg 25 µg Biotin block buffer 1 x 500µl 1 x 250µl Streptavidin Alexa Fluor® 647 (Monovalent) reagent 1 x 500µl 1 x 250µl
