Overview
Product name
MitoBiogenesis™ Western Blot CocktailSpecies reactivity
Reacts with:Mouse, Rat, Cow, HumanProduct overview
The MitoBiogenesis™ western blotting cocktail (ab123545) is designed to study the regulation of mitochondrial biogenesis and cellular stress in response to environmental stimuli. It also can be used to monitor drug-induced effects on mitochondrial biogenesis. The two main components of this cocktail target two proteins, which are each subunits of a different oxidative phosphorylation enzyme complex, one subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. An anti-Beta actin antibody is used as loading control.
Cocktail Antibodies:
Mouse CII-70 (SDHA) [2E3GC12FB2AE2] monoclonal:
Amount: 25 µg
Working concentration: 0.5 µg/ml
Mouse CIV-1 (MT-CO1) [1D6E1A8] monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse Beta actin [mAbcam 8224] monoclonal:
Amount: 25 µg
Working concentration: 0.5 µg/ml
Notes
Antibody Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Related products
Review themitochondrial assay guide, or the fullmetabolism assay guideto learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Tested applications
Suitable for:WBmore details
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 100 µg Antibody Cocktail 1 x 200µl Research areas
- Signal Transduction
- Cytoskeleton / ECM
- Cytoskeleton
- Microfilaments
- Actin etc
- Actin
- Signal Transduction
- Signaling Pathway
- Lipid Signaling
- Prostaglandins
- Signal Transduction
- Metabolism
- Energy Metabolism
- Signal Transduction
- Metabolism
- Mitochondrial
- Signal Transduction
- Metabolism
- Lipid metabolism
- Cell Biology
- Other Antibodies
- Oxidative Stress
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Metabolism of carbohydrates
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Integration of energy metabolism
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Mitochondrial markers
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Carbohydrate metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Metabolism
- Pathways and Processes
- Redox metabolism
- Oxidative stress
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Oxidative phosphorylation
- Complex II
- Metabolism
- Types of disease
- Cancer
- Kits/ Lysates/ Other
- Kits
- Antibody Cocktails
- MitoBiogenesis
Database links
- SwissProt:P23219 Human
- SwissProt:P60709 Human
- SwissProt:P31040 Human
